アブストラクト（32巻2号：The Bulletin of Kanagawa Dental College）

The Bulletin of Kanagawa Dental College

English

Title　： Distribution and Characterization of Porphyromonas gulae
Subtitle　： Selective Proceedings of 38th General Meeting of Kanagawa Odontological Society, 2003
Authors　： Nobushiro Hamada1, Hidefumi Kumada1, Takehiko Hiyama2, Ko Nakajima2, Sadao Sato2, Toshio Umemoto1
Authors（kana）　：
Organization　： 1Department of Infection Control, Kanagawa Dental College, 2Department of Craniofacial Growth and Development Dentistry, Kanagawa Dental College
Journal　： The Bulletin of Kanagawa Dental College
Volume　： 32
Number　： 2
Page　： 104-106
Year/Month　： 2004 / 9
Article　： Report
Publisher　： Kanagawa Odontological Society
Abstract　： A 16S rRNA-based PCR detection method was used to determine the prevalence of Porphyromonas gingivalis and Porphyromonas gulae in subgingival specimens from dogs and humans. P.gingivalis only detected the specimens from humans. However, P.gulae detected the specimens from dogs and humans. The fimbrial protein was purified from Porphyromonas gulae ATCC 51700 by DEAE-Sepharose CL-6B column chromatography. The fimbrial protein was eluted at 0.15 M NaCl, and the molecular mass of this protein was approximately 41K as estimated by SDS-PAGE. Immunogold electron microscopy showed that the anti-41K fimbriae serum bound to the fimbria on the cell surface of P.gulae. The component of the fimbrial structure (fimbrillin) of P.gulae ATCC 51700 was the same in size and in antigenicity as that of 41K major fimbrial protein of P.gingivalis ATCC 33277. In addition, the presence of P.gulae was also found in human oral cavity.
Practice　： Dentistry
Keywords　： Porphyromonas gulae, Porphyromonas gingivalis, fimbriae, 41K protein