アブストラクト（32巻2号：The Bulletin of Kanagawa Dental College）

The Bulletin of Kanagawa Dental College

English

Title　： Isolation of cementobast progenitor from immortalized bovine dental follicle cells.
Subtitle　： Selective Proceedings of 38th General Meeting of Kanagawa Odontological Society, 2003
Authors　： Masahiro Saito, Akira Tsunoda, Takanori Tsubakimoto, Toshio Teranaka
Authors（kana）　：
Organization　： Department of Operative Dentistry and Endodontics Kanagawa Dental College
Journal　： The Bulletin of Kanagawa Dental College
Volume　： 32
Number　： 2
Page　： 113-116
Year/Month　： 2004 / 9
Article　： Report
Publisher　： Kanagawa Odontological Society
Abstract　： The dental follicle is the mesenchymal tissue surrounding the developing tooth germ. During tooth root development, progenitor cells present in the dental follicle are believed to play a central role in the formation of periodontal components (cementum, periodontal ligament and alveolar bone). However, little more is known about the biology of these progenitors. Previously, we observed that cultured bovine dental follicle cells (BDFC) contained putative cementoblast progenitors. To further analyze the biology of these cells, we attempted to immortalize BDFC by expression of the polycomb group protein, Bmi-1, and human telomerase reverse transcriptase (hTERT). BDFC expressing Bmi-1 and hTERT demonstrated an extended life span - 90 population doublings more than normal BDFC - and still contained cells with potential to differentiate into cementoblasts upon implantation into immunodeficient mice. From these cells, we established a clonal cell line, designated BCPb8, which formed cemetum-like mineralized tissue reactive to anti-cementum specific monoclonal antibody, 3G9, and expressed mRNA for bone sialoprotein, osteocalcin, osteopontin and type I collagen upon implantation. Thus, using Bmi-1 and hTERT, we succeeded in immortalizating cementoblast progenitor cells from BDFC without affecting differentiation potential. The BCPb8 cell line being the first established immortalized clonal cell lines of cementoblast progenitor, could be a useful tool not only to study cementogenesis but also to develop a regeneration therapy for patients with periodontitis.
Practice　： Dentistry
Keywords　： Dental follicle, Immortalizationn, cementoblast Progenitor, Differentiation