アブストラクト(32巻2号:The Bulletin of Kanagawa Dental College)

The Bulletin of Kanagawa Dental College

English

Title : Studies on in vivo Measurement of Oxidative Stress in Experimentally-Induced Arthritis of the TMJ
Subtitle : Selective Proceedings of 38th General Meeting of Kanagawa Odontological Society, 2003
Authors : Yoshiaki Kawai1, Hirofumi Shoji2, Hiroyuki Miyazaki1, Hiroharu Katoh1, Masako Suga1, Masaichi (Chang-il) Lee2, Eiro.Kubota1
Authors(kana) :
Organization : 1Department of Oral and Maxillofacial Surgery, Kanagawa Dental College, 2Department of Pharmacology and ESR Laboratory, Kanagawa Dental College
Journal : The Bulletin of Kanagawa Dental College
Volume : 32
Number : 2
Page : 136-139
Year/Month : 2004 / 9
Article : Report
Publisher : Kanagawa Odontological Society
Abstract : In the present study, we evaluated the oxidative stress of the temporomandibular joint (TMJ) region by using in vivo L-band ESR. Rat TMJ arthritis model was experimentally induced by injection with the rat recombinant IL-1β or TNF-α into the TMJ. We used a 3-carbamoy1-2,2,5,5-tetramethylpyrrolidine-1-yloxy (C-PROXYL) as a nitrooxide spin probe, and performed the measurement of signal decay rate of C-PROXYL in the TMJ region by using in vivo L-band ESR. Results showed that the decay rate of C-PROXYL non-invasively measured by L-band ESR in cytokine induced arthritic TMJ groups were much higher than that in the control group. We also succeeded in reconstructing 3D images of the distribution of C-PROXYL in the TMJ superior joint space of the live animal non-invasively. We could measure the oxidative stress in the cytokine induced rat TMJ arthritis model by L-band ESR. With further advancement in in vivo ESR technique, this technology holds great promise for non-invasive assessment of oxidative stress in the temporomandibular disease (TMD).
Practice : Dentistry
Keywords : Temporomandibular joint, Oxidative stress, Free radical, Electron spin resonance, In vivo