アブストラクト（33巻2号：The Bulletin of Kanagawa Dental College）

The Bulletin of Kanagawa Dental College

English

Title　： Invasive Porphyromonas gingivalis infection modulates expression of atherogenic molecules in human aortic endothelial cells
Subtitle　： Selective Proceedings of 39th General Meeting of Kanagawa Odontological Society, 2004
Authors　： Yusuke Takahashi, Toshio Umemoto
Authors（kana）　：
Organization　： Department of Oral Microbiology, Kanagawa Dental College
Journal　： The Bulletin of Kanagawa Dental College
Volume　： 33
Number　： 2
Page　： 133-135
Year/Month　： 2005 / 9
Article　： Report
Publisher　： Kanagawa Odontological Society
Abstract　： [ABSTRACT] Invasive property of Porphyromonas gingivalis is often characterized by expression of fimbriae. To analyze function of the fimbriae on cellular responses of human aortic endothelial cells (HAEC) caused by P.gingivalis infection, the minor fimbria gene was inactivated in P.gingivalis wildtype strain 381 and in a major fimbria deficient strain (DPG3). Among the mutants, DPG3, 381MF1 (possesses only major fimbriae), DPGMFB (fimbriae null mutant) and the wildtype strain, invasion property was major fimbria-dependent. Invasion was associated with the stimulation of IL-1β, IL-8, MCP-1 and surface cellular adhesion molecules (CAMs). Stimulation of HAEC with purified major or minor fimbriae also induced IL-8, MCP-1 and CAMs. Pretreatment of HABC with cytochalasin D disrupted all the HAEC responses. These data indicate that stimulation by both fimbriae can induce atherogenic response in HAEC through cytoskeltal rearrangement, although only major fimbria is deeply related to invasion of P.gingivalis.
Practice　： Dentistry
Keywords　： cardiovascular disease, Porphyromonas gingivalis, endothelial cells, fimbriae