アブストラクト（33巻2号：The Bulletin of Kanagawa Dental College）

The Bulletin of Kanagawa Dental College

English

Title　： Characterization of immortalized mouse dental follicle cells
Subtitle　： Selective Proceedings of 39th General Meeting of Kanagawa Odontological Society, 2004
Authors　： Takamasa Yokoi1),2), Masahiro Saito1), Eisaku Nishida1),2), Kazutaka Kosaka1), Takanori Tsubakimoto1), Toshio Teranaka1)
Authors（kana）　：
Organization　： 1)Department of Operative Dentistry and Endodontics, Kanagawa Dental College, 2)Department of Periodontology, School of Dentistry, Aichi-Gakuin University
Journal　： The Bulletin of Kanagawa Dental College
Volume　： 33
Number　： 2
Page　： 136-140
Year/Month　： 2005 / 9
Article　： Report
Publisher　： Kanagawa Odontological Society
Abstract　： [ABSTRACT] Dental follicle is the mesechymal tissue which surround developing tooth germ. During tooth root development, periodontal components such as cementum, periodontal ligament and alveolar bone are created by progenitors present in the dental follicle. Here, we report the presence of periodontal ligament progenitor cell line in mice dental follicle cells (MDF). MDF were obtained from mice tooth germ, and immortalized by expression of mutant human papilloma virus type 16 E6 gene lacking PDZ domain. MDF expressing mutant E6 gene extended life span beyond 150 population doublings, in contrast normal MDF failed to proliferate until 10 population doublings. Immortalized MDF expressed tendon phenotype-related gene such as Scleraxis (Scx), Growth and differentiation factor (GDF)-5, EphA4 and Six-1 and type I collagen. In addition to the tendon phenotype related gene, expression of osteopontin and periostin were observed. To elucidate the differentiation capacity of immortalized MDF in vivo, cells were transplanted into severe combined immunodeficiency (SCID) mice and analyzed after 4 weeks. Transplanted immortalized MDF formed periodontal ligament-like tissue which is deposited oxytaran fiber-like microfibrils, and no such characteristic morphology was observed in the control transplants by using NIH3T3. Our findings suggested that immortalized MDF possessed periodontal ligament progenitor properties, and may provide an useful information not only for development of periodontal ligament but also develop a regeneration therapy that affect patient with periodontal disease.
Practice　： Dentistry
Keywords　： Dental follicle, Immortalization, Periodontal ligament progenitor, Differentiation