アブストラクト（34巻2号：The Bulletin of Kanagawa Dental College）

The Bulletin of Kanagawa Dental College

English

Title　： Establishment of gene expression profiling database from human periodontal ligament.
Subtitle　： Selective Proceedings of 40th General Meeting of Kanagawa Odontological Society, 2005
Authors　： Eisaku Nishida1,2,3, Masahiro Saito1,2, Takamasa Yokoi1,2,3, Takanori Tsubakimoto1,2, Kazutaka Kosaka1,2, Makoto Aino1,2,3, Toshio Teranaka1
Authors（kana）　：
Organization　： 1Department of Operative Dentistry and Endodontics, Kanagawa Dental college, 2Oral Health Science Research Center, 3Department of Periodontology, School of Dentistry, Aichi-Gakuin University
Journal　： The Bulletin of Kanagawa Dental College
Volume　： 34
Number　： 2
Page　： 109-111
Year/Month　： 2006 / 9
Article　： Report
Publisher　： Kanagawa Odontological Society
Abstract　： Development of periodontal ligament(PDL) occurred as a series of events that requires temporal and spatial expression of numerous genes regulated differentiation of dental follicle(DF) cells. Nevertheless the genes required for PDL development have not been identified yet. To solve this problem, we used in silico approach with a human PDL database of expressed sequence tags(ESTs) named "periome" database. ESTs were obtained by partially sequencing of 5'end of 10,000 cDNA clones from human PDL cDNA library, and identified 4,384 unique EST clusters. 617 EST clusters appearing more than 3 times were collected, and classified by functional annotation to produce periome database. From periome database, we screened DF specific EST clusters by in situ hybridization. The result showed that SPARC like 1, Nidogen 1, Spondin 1 specifically expressed in DF. From these findings, periome database was successfully established, and this database provides a transcriptome resource fer analyzing gene clusters involved in PDL development.
Practice　： Dentistry
Keywords　： Periodontal ligament t, ESTs, cDNA library, Expression profiling