アブストラクト(34巻2号:The Bulletin of Kanagawa Dental College)

The Bulletin of Kanagawa Dental College

English

Title : Establishment of dental follicle cells culture system that generating periodontal ligament in vivo
Subtitle : Selective Proceedings of 40th General Meeting of Kanagawa Odontological Society, 2005
Authors : Kazutaka Kosaka1,2, Takamasa Yokoi1,2,3, Masahiro Saito1,2, Eisaku Nishida1,2,3, Takanori Tsubakimoto1,2, Makoto Aino1,2,3, Toshio Teranaka1
Authors(kana) :
Organization : 1Department of Operative Dentistry and Endodontics, Kanagawa Dental Collegel, 2Oral Health Science Research Center, Kanagawa Dental College, 3Department of Periodontology, School of Dentistry, Aichi-gakuin University
Journal : The Bulletin of Kanagawa Dental College
Volume : 34
Number : 2
Page : 112-114
Year/Month : 2006 / 9
Article : Report
Publisher : Kanagawa Odontological Society
Abstract : The dental follicle is a mesechymal tissue that surrounds the developing tooth germ. During tooth root formation, periodontal components (cementum, periodontal ligament[PDL] and alveolar bone) are created by dental follicle progenitors. Here, we report the presence of PDL progenitors in mice dental follicle (MDF) cells. MDF cells were obtained from mice incisor tooth germs, and immortalized by expression of a mutant human papilloma virus type 16 E6 gene lacking the PDZ domain-binding motif. MDF cells expressing mutant E6 gene (MDFE6-EGFP cells) had an extended life span, beyond l50 population doublings (PD). In contrast, normal MDF cells failed to proliferate beyond 10 PD. MDFE6-EGFP cells expressed tendon/ligament phenotype-related genes such as Scleraxis(Scx), grouwth and differentiation factor (GDF)-5, EphA4, Six-1 and type I collagen. In addition, expression of PDL-specific gene, such as periostin, was observed. To elucidate the differentiation capacity of MDFE6-EGFP cells in vivo, cells were transplanted into severe combined immunodeficiency (SCID) mice. At 4 weeks, MDFE6-EGFP cell transplants had the capacity to generate a PDL-like tissue with strong expression of periostin, and fibrillar assembly of type I collagen. Our findings suggest that PDL progenitors are present in MDFE6-EGFP cells, and these cells may provide useful information for the formation of PDL for research purposes and for the development of regeneration therapies.
Practice : Dentistry
Keywords : Dental follicle, Regeneration, periodontal ligament Progenitor, Immortalization, Differentiation