アブストラクト（34巻2号：The Bulletin of Kanagawa Dental College）

The Bulletin of Kanagawa Dental College

English

Title　： Establishment of dental papilla cells cuiture system that can differentiation into odontoblast.
Subtitle　： Selective Proceedings of 40th General Meeting of Kanagawa Odontological Society, 2005
Authors　： Takanori Tsubakimoto1,2, Masahiro Saito1,2, Takamasa Yokoi3, Eisaku Nishida2,3, Kazutaka Kousaka1,2, Makoto Aino2,3, Toshio Teranaka1,2
Authors（kana）　：
Organization　： 1Department of Operative Dentistry and Endodontics, Kanagawa Dental College, 2Oral Health Science Research Center, 3Department of Periodontology, School of Dentistry, Aichi-gakuin University
Journal　： The Bulletin of Kanagawa Dental College
Volume　： 34
Number　： 2
Page　： 115-117
Year/Month　： 2006 / 9
Article　： Report
Publisher　： Kanagawa Odontological Society
Abstract　： Dental papilla cells derived from cranial neural crest-derived mesechymal progenitors, and act as a reservoir for odontoblast progenitors during tooth germ development. Differentiation of dental papilla cells seems to be regulated by epithelial-mesenchymal interaction. However biological roles of these cells remain largely unknown. To address these, we attempt to establish immortalized mouse dental papilla cells (MDPE&) , and investigate if they possessed odontoblast progenitor, MDPE6 was continuous grown beyond population doubling 80 without changing cell morphology and proliferation rate. MDPE6 showed a high mineralization potential with expression of osteoblast marker genes. However, no expression was observed odontoblast marker gene such as dentin sialoprotein (DSP) in this condition. MDPE6 is capable of differentiating into odontoblast with DSP expression when cultured on matrigel. Our result suggests that MDPE6 possessed odontoblast progenitors, and basement membrane components could be regulated differentiation of these cells. Thus, MDPE6 provide a useful cell culture system for investigating molecular mechanisms of odontogenesis.
Practice　： Dentistry
Keywords　： Dental papilla cell, odontoblast progenitor, Dentin sialoprotein (DSP)