アブストラクト(36巻2号:The Bulletin of Kanagawa Dental College)

The Bulletin of Kanagawa Dental College

English

Title : Noninvasive Measurement of Oxidative Stress in Experimentally Induced Arthritis of the Temporomandibular Joint using in vivo Electron Spin Resonance Spectroscopy
Subtitle : ORIGINAL ARTICLES
Authors : Dai-taro Tajima1), Yoshiaki Kawai1), Fumihiko Yoshino2), Masaichi-Chang-il Lee2), Eiro Kubota1)
Authors(kana) :
Organization : 1)Department of Oral and Maxillofacial Surgery, Kanagawa Dental College, 2)Department of Clinical Care Medicine, Division of Pharmacology and ESR Lahoratories, Kanagawa Dental College
Journal : The Bulletin of Kanagawa Dental College
Volume : 36
Number : 2
Page : 47-53
Year/Month : 2008 / 9
Article : Original article
Publisher : Kanagawa Odontological Society
Abstract : [Abstract] In the present study, we evaluated oxidative stress in the temporomandibular joint (TMJ) using an in vivo L-band electron spin resonance (ESR) technique. The TMJ arthritis was experimentally induced in rats with injections of rat recombinant IL-β or TNF-α in the TMJ. We used a 3-carbamoyl-2, 2, 5, 5-tetramethylpyrrolidine-1-yloxy (C-PROXYL) as a nitrooxide spin probe, and measured the signal decay rate of C-PROXYL in the TMJ region using an L-band ESR. The results showed that the decay rates of C-PROXYL noninvasively measured with an L-band ESR in cytokine induced arthritic TMJ groups were much higher than those in the control group. We also found that the decay rate of the IL-β-injected arthritic group was much faster than that of the control and the TNF-α-injected groups at 12h post injection. Whereas the decay rate of the TNF-α-injected arthritis group was much faster after 48h pos-tinjection than that of the control and the lL-β-injected groups, the decay rates of both the IL-β and TNF-α groups were at the same level after seven days postinjection. These results may imply that IL-1β is capable of inducing inflammatory changes as well as of producing reactive oxygen species (ROS) more rapidly than TNF-α in rat TMJ, but these changes are transient and they had recovered by the seventh day after the cytokine administration. Thus, we were able to measure the fine changes in oxidative stress caused by ROS in the cytokine induced rat TMJ arthritis using an L-band ESR. With further advancement in in vivo ESR techniques, this technology holds great promise for the noninvasive assessment of oxidative stress in temporomandibular disorders (TMD).
Practice : Dentistry
Keywords : Temporomandibular joint (TMJ), Oxidative stress, Reactive oxygen species (ROS), IL-β, TNF-α, L-band electron spin resonance (ESR)