アブストラクト（36巻2号：The Bulletin of Kanagawa Dental College）

The Bulletin of Kanagawa Dental College

English

Title　： Deficiency of a Gelatinase Alters Osteocyte Functions
Subtitle　： Selective Proceedings of 42nd General Meeting of Kanagawa
Authors　： Takami Furuhama1), Shigeyoshi Itohara2), Yuko Mikuni-Takagaki1)
Authors（kana）　：
Organization　： 1)Department of Functional Biology, Kanagawa Dental College, 2)Laboratory for Behavioral Genetics, RIKEN BSI
Journal　： The Bulletin of Kanagawa Dental College
Volume　： 36
Number　： 2
Page　： 84-86
Year/Month　： 2008 / 9
Article　： Report
Publisher　： Kanagawa Odontological Society
Abstract　： [Abstract] We have reported that mutations of the gene encoding MMP-2, an extracellular matrix (ECM) degrading gelatinase, decrease bone mineral density (BMD) in long bones, but increase bone volume in calvaria. In the long bones, the osteocytic networks were disrupted and the bone density kept decreasing throughout life, while osteoblast and osteoclast functions appeared normal. To evaluate the mechanical factors in different responses of long bones and calvaria, we isolated MMP2-/- osteoblast and osteocytes from 16-week mice and analyzed mRNAs by quantitative PCR. In the expression of certain genes, MMP2-/- long bone osteocytes responded to stretching in an entirely different manner compared to the wild type counterparts. Our results suggested that a significantly increased level of inhibitory sclerostin gene expressed in MMP2-/- long bone osteocytes reduced the activity of osteoblasts resulting in the loss of bone mass.
Practice　： Dentistry
Keywords　： MMP2, osteocyte, osteoblasts, mechaniical response, BMD