アブストラクト（37巻2号：The Bulletin of Kanagawa Dental College）

The Bulletin of Kanagawa Dental College

English

Title　： Matrix Metalloproteinase-2 and Bone Formation
Subtitle　： Selective Proceedings of 43rd General Meeting of Kanagawa Odontological Society, 2008
Authors　： Takami Furuhama*1, Yuko Mikuni-Takagaki*2
Authors（kana）　：
Organization　： *1Kanagawa Dental College, *2Department of Functional Biolog y, Kanagawa Dental College
Journal　： The Bulletin of Kanagawa Dental College
Volume　： 37
Number　： 2
Page　： 134-136
Year/Month　： 2009 / 9
Article　： Report
Publisher　： Kanagawa Odontological Society
Abstract　： [Abstract] In a mouse with a deficient gene encoding MMP-2, an extracellular matrix (ECM) degrading gelatinase, functions of osteoblast and osteoclast appeared normal. However, decreased bone mineral density (BMD) and cortical thickness in adult long bones as well as the increased bone volume in adult calvaria were the features of the knock out mouse. In these knock out bones, the osteocytic networks were disrupted. Among the isolated Mmp2-/- osteoblast and osteocytes, the Mmp2-/- long bone osteocyte responded to stretching in an entirely opposite manner to the wild type cells by expressing the increased level of sclerostin. Bone quality assessed by Raman spectroscopy suggested that the locally increased level of sclerostin expressed in the Mmp2-/- osteocytes inactivate bone formation by osteoblasts resulting in the loss of bone mass in the Mmp2-/- long bone. [Introduction] ECM production and degradation by bone cells are critical steps in bone metabolism. The turnover of Type I collagen, a major ECM component, is especially important and mutations in type I collagen gene cause osteogenesis imperfecta.
Practice　： Dentistry
Keywords　： MMP-2, osteocyte, osteoblast, mechanical response, bone formation