アブストラクト（38巻2号：The Bulletin of Kanagawa Dental College）

The Bulletin of Kanagawa Dental College

English

Title　： Mechanical Response of Mandibular osteocytes
Subtitle　： Selective Proceedings of 44th General Meeting of Kanagawa Odontological Society, 2009
Authors　： Takafumi Furuhama1), Hirotaka Watabe2), Nubuyuki Tani-Ishii2), Yuko Mikuni-Takagaki1)
Authors（kana）　：
Organization　： 1)Department of Functional Biology, Kanagawa Dental College, 2)Division of Endodontic, Kanagawa Dental College
Journal　： The Bulletin of Kanagawa Dental College
Volume　： 38
Number　： 2
Page　： 79-81
Year/Month　： 2010 / 9
Article　： Report
Publisher　： Kanagawa Odontological Society
Abstract　： [ABSTRACT] While osteonecrosis of a jaw (ONJ) has been characterized as complications secondary to Bisphosphonate (BP) therapy, it is now associated with pharmaceutical agents other than bisphosphonates. In a recent, large randomized phase III clinical trials of denosumab, an anti-RANKL agent, to prevent fractures and bone loss in cancer patients with bone metastases, ONJ appeared at a higher rate than with zerodronic acid, a bisphosphonate. It remains largely unknown why ONJ occurs only in mandibular bone. In order to evaluate the role of mechanical factors in different apoptotic response of mandibular osteocytes compared with the cells of long bone and calvarial origin, we isolated osteocytes from C57B6J mouse bones, stretched them after one-week culture and analyzed mRNAs and proteins by quantitative RT-PCR and immunofluorescence staining, respectively. In the expression of genes related to bone resorption such as RANKL, mandibular osteocytes responded to stretching in an entirely different manner from long bone and calvarial cells. Our results suggested that tridermic origin as well as the mechanical environment is important in determining cellular response to the anti-resorptive agents.
Practice　： Dentistry
Keywords　： osteocytes, mandible, osteonecrosis of jaw (ONJ), mechanical response, bone formation