アブストラクト（38巻2号：The Bulletin of Kanagawa Dental College）

The Bulletin of Kanagawa Dental College

English

Title　： Chemokine CXCL14 / BRAK transgenic mice suppress growth of carcinoma cell xenografts
Subtitle　： Selective Proceedings of 44th General Meeting of Kanagawa Odontological Society, 2009
Authors　： Kazuhito Izukuri1),2), Kenji Suzuki1),3), Nobuyuki Yajima1),2), Shigeyuki Ozawa1),3), Shin Ito1),2), Eiro Kubota1),3), Ryu-Ichiro Hara1),2)
Authors（kana）　：
Organization　： 1)Oral Health Science Research Center, 2)Department of Biochemistry and Molecular Biology, 3)Department of Oral and Maxillofacial Surgery, Kanagawa Dental College
Journal　： The Bulletin of Kanagawa Dental College
Volume　： 38
Number　： 2
Page　： 82-84
Year/Month　： 2010 / 9
Article　： Report
Publisher　： Kanagawa Odontological Society
Abstract　： [ABSTRACT] We reported previously that the forced expression of the chemokine BRAK, also called CXCL14 in head and neck squamous cell carcinoma (HNSCC) cells decreased the rate of tumor formation and size of tumor xenografts compared with mock-vector treated cells in athymic nude mice or in severe combined immunodeficiency mice. This suppression occurred even though the growth rates of these cells were the same under in vitro culture conditions, suggesting that a high expression level of the gene in tumor cells is important for the suppression of tumor establishment in vivo. The aim of this study was to determine whether CXCL14/BRAK transgenic mice show resistance to tumor cell xenografts or not. CXCL14/BRAK cDNA was introduced into male C57BL/6J pronuclei, and 10 founder transgenic mice (Tg) were obtained. Two lines of mice expressed over 10 times higher CXCL14/BRAK protein levels (14 and 11 ng/ml plasma, respectively)than normal blood level (0.9 ng/ml plasma), without apparent abnormality. The sizes of Lewis lung carcinoma and B16 melanoma cell xenografts in Tg mice were significantly smaller than those in control wild-type mice, indicating that CXCL14/BRAK, first found as a suppressor of tumor progression of HNSCC, also suppresses the progression of carcinomas of other tissue origins. Immunohistochemical studies showed that invasion of blood vessels into tumors was suppressed in tumor xenografts of CXCL14/BRAK Tg mice. These results indicate that CXCL14/BRAK suppressed tumor cell xenografts by functioning paracrine or endocrine fashion and that CXCL14/BRAK is a very promising molecular target for tumor suppression without side effects.
Practice　： Dentistry
Keywords　： Chemokine CXCL14/BRAK, Tumor suppression, Lewis lung Carcinoma cells, Transgenic mouse, Tumor therapy