アブストラクト（39巻2号：The Bulletin of Kanagawa Dental College）

The Bulletin of Kanagawa Dental College

English

Title　： Mechanical loading dependent metabolic response in mandibular osteoblasts
Subtitle　： Selective Proceedings of 45th General Meeting of Kanagawa Odontological Society, 2010
Authors　： Hirotaka Watabe*1, Takami Furuhama*2, Yuta Funayama*2, Nobuyuki Tani-Ishii*1, Yuko Mikuni-Takagaki*2
Authors（kana）　：
Organization　： *1Division of Endodontics, Department of Oral medicine, Kanagawa Dental College, *2Division of Biochemistry, Department of Functional Biology, Kanagawa Dental College
Journal　： The Bulletin of Kanagawa Dental College
Volume　： 39
Number　： 2
Page　： 117-120
Year/Month　： 2011 / 9
Article　： Report
Publisher　： Kanagawa Odontological Society
Abstract　： [ABSTRACT] Therapies with bisphosphonates as well as with anti-RANKL monoclonal antibody have been suspected to increase the risk of osteonecrosis of the jaw. The details of the pathogenic mechanism of the disease have yet to be determined. The mandible is unique in being exposed to large occlusal force, which causes a high turnover rate of mandibular bone. In order to study mechanisms how mechanical force increases turnover in the mandibular bone, we compared responses to the low intensity pulsed ultrasound (LIPUS) among the mouse osteoblasts derived from bones of different sites. We prepared osteoblasts from the mandible, calvaria and long bones of C57B6J mice, incubated for one week, and harvested them after two 20-min LIPUS exposure separated by an interval of 24 hours. Messages were analyzed by real-time RT-PCR and proteins were identified by Western blotting followed by immunofluorescent staining. Cell differentiation and enhanced Wnt/β-catenin pathways were similarly observed as a result of LIPUS exposure in all osteoblast types. In mandibular cells, both RANKL mRNA and protein levels behaved in a manner different from other types. The basal level, which was significantly lower than the levels in long bone and calvarial osteoblasts, did rise after LIPUS exposure to the level comparable to others. LIPUS also affected apoptosis-related protein levels in mandibular osteoblasts in an anti-apoptotic manner. It is suggested that mandibular osteoblasts plays an essential role in remodeling, which uniquely depends on mechanical force that is applied to mandibular bone.
Practice　： Dentistry
Keywords　： Tridermic origin, Osteoblast, Remodeling, Mechanical loading