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アブストラクト（20巻4号：神奈川歯学）
Japanese
| Title ： | 培養腫瘍細胞におけるGa-67の取り込みとATP代謝 |
|---|---|
| Subtitle ： | 原著 |
| Authors ： | 小林雅人 |
| Authors（kana） ： | |
| Organization ： | 神奈川歯科大学放射線学教室 |
| Journal ： | 神奈川歯学 |
| Volume ： | 20 |
| Number ： | 4 |
| Page ： | 439-448 |
| Year/Month ： | 1986 / 3 |
| Article ： | 原著 |
| Publisher ： | 神奈川歯科大学学会 |
| Abstract ： | 「緒言」67Ga-citrate(以下67Gaと略す)は現在, 悪性腫瘍および急性炎症の病巣の画像診断に広く利用されている. しかし, 67Gaが細胞に取り込まれるメカニズムは, 現在も完全に解明されていない. 1970年, Hayesら, 1972年, Bichelらは, 67Gaの悪性腫瘍への取り込みは, 変性もしくは壊死の組織よりも活動性の組織に多く取り込まれ, 細胞の増殖と関係があるらしいと報告した. 1984年, 当教室の山口は, 培養腫瘍細胞を用いて, 67Gaの取り込みが細胞周期のS期(DNA合成期)で最も多くなることを報告した. 一方, 1978年, Ishiguroら1984年, Weismanらは, DNA合成にATPが重要な役割をはたしていると述べている. そこで今回, 著者は培養腫瘍細胞を用いて, 細胞内のATP代謝と67Gaの取り込みの関係を検討した. |
| Practice ： | 歯科学 |
| Keywords ： |
English
| Title ： | Experimental study on Ga-67 uptake and ATP metabolism in cultured tumor cells |
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| Subtitle ： | Original article |
| Authors ： | Masato KOBAYASHI |
| Authors（kana） ： | |
| Organization ： | Department of Radiology, Kanagawa Dental College |
| Journal ： | Kanagawa Shigaku |
| Volume ： | 20 |
| Number ： | 4 |
| Page ： | 439-448 |
| Year/Month ： | 1986 / 3 |
| Article ： | Original article |
| Publisher ： | Kanagawa Odontological Society |
| Abstract ： | In order to clarify the molecular mechanism of Ga-67 uptake in malignant tumor cells, the effects of NaF and ouabain on Ga-67 uptake in mouse leukemia cells were examined. The uptake of Ga-67 in control cells had gradually increased during incubation with a concomitant cell proliferation. However, when NaF was added to these cells suspensions, Ga-67 uptake did not increase and kept a constant level, and ATP content of these NaF treated cells was much lower than that of control cells, because NaF is a potent inhibitor of glycolysis. Therefore the process of Ga-67 uptake in these cells is considered to be dependent on intracellular ATP content. Then the effect of ouabain, a specific inhibitor of Na+ -K+ -ATP ase in the plasmamembrane, on Ga-67 uptake was investigated. After addition of ouabain to them, not only a cell proliferation but also Ga-67 uptake was strongly inhibited, although ATP content of these ouabain treated cells had slightly increased as compared with control cells. Overall, these results strongly suggest that the process of Ga-67 uptake is ATP dependent and that Na+ -K+ -ATP ase plays an important role in Ga-67 uptake. Further, when Fe-59 was used in stead of Ga-67, similar results were obtained. The accumulation of Ga-67 seemed to closely resemble that of Fe-59, indicating that there was a close correlation between Ga-67 and Fe-59. |
| Practice ： | Dentistry |
| Keywords ： |
