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アブストラクト（24巻3号：神奈川歯学）
Japanese
| Title ： | Actinobacillus actinomycetemcomitans 細胞壁 ペプチドグリカンの化学構造と免疫調節活性 |
|---|---|
| Subtitle ： | 原著 |
| Authors ： | 木下藤朗, 梅本俊夫 |
| Authors（kana） ： | |
| Organization ： | 神奈川歯科大学口腔細菌学教室 |
| Journal ： | 神奈川歯学 |
| Volume ： | 24 |
| Number ： | 3 |
| Page ： | 523-535 |
| Year/Month ： | 1989 / 12 |
| Article ： | 原著 |
| Publisher ： | 神奈川歯科大学学会 |
| Abstract ： | 「緒言」歯周ポケット内細菌叢の生態学的研究から異なる病型の歯周病には, それぞれ特異的な細菌あるいは細菌群が関与していることが指摘されている. また, 歯周炎病巣に認められる組織破壊が, 免疫病理学的機序によって誘起される可能性のあることなどが報告されている. すなわちNewman(1976), Slots(1979)およびTanner(1979)らは, さまざまな病型の歯周病患者の歯周ポケット内細菌叢を健全者のそれと比較して, グラム陰性嫌気性桿菌の占める割合が著しく高いことを指摘するとともに, 若年性歯周炎(Juvenile periodontitis)ではActinobacillus actinomycetemcomitansとCapnocytophaga species, 急速進行性歯周炎(Rapidly progressive periodontitis)では, 主としてBacteroides gingivalis, Fusobacterium nucleatumおよび口腔スピロヘータ, さらに成人性歯周炎(Adult periodontitis)では, B.gingivalisあるいはBacteroides intermedius, Eikenella corrodens, そして口腔スピロヘーターなどが高率に検出されることを明らかにしている. |
| Practice ： | 歯科学 |
| Keywords ： | アクチノバチルス, ペプチドグリカン, 免疫調節活性 |
English
| Title ： | Chemical Structure and Immunomodulating Activities of Peptidoglycan from Actinobacillus actinomycetemcomitans |
|---|---|
| Subtitle ： | Original article |
| Authors ： | Fujio KINOSHITA, Toshio Umemoto |
| Authors（kana） ： | |
| Organization ： | Department of Oral Microbiology, Kanagawa Dental College |
| Journal ： | Kanagawa Shigaku |
| Volume ： | 24 |
| Number ： | 3 |
| Page ： | 523-535 |
| Year/Month ： | 1989 / 12 |
| Article ： | Original article |
| Publisher ： | Kanagawa Odontological Society |
| Abstract ： | Abstracts : The chemical structure and immunomodulating activities of the cell wall peptidoglycans isolated from Actinobacillus actinomycetemcomitans were investigated. Peptidoglycans were isolated from A. actinomycetemcomitans strains Y4 and ATCC 29522 by boiling in 4% sodium dodecyl sulfate and by digestion with pronase, trypsin and α-amylase. Analysis of amino acids and amino sugars of the peptidogylcans revealed that glucosamine, muramic acid, D-glutamic acid, D-alanine, and meso-2, 6-diaminopimelic acid (A2pm) were the principal components. Serine and glycine were not found. Dinitrophenylation method revealed that about half of A2pm residue had a free aminogroup, and analysis by hydrazinolysis showed that a small part of alanine and A2pm located at the C-terminal. The above results indicate that one of the amino groups of A2pm residue at one strand of the stem peptide subunit crosslinked to the carboxyl group of alanine of the neighboring strand. It was thus reveled that the peptidoglycans of A.actinomycetemcomitans belonged to the A1γ type of the classification by Schleifer and Kandler. Peptidoglycans isolated from A.actinomycetemcmitans strain Y4 and ATCC 29522 were found to be definitely adjuvant-active in induction of delayed type hypersensitivity against ovalbumin when administered to guinea pigs as water-in oil emulsion and stimulation of increase serum antibody levels was found in both peptidoglycans. Regarding mitogenicity on splenocytes of BALB/c and BALB/c nu/nu mice, peptidoglycans from two strains of A.actinomycetemcomitans were markedly enhanced the uptake〔3H〕thymidine in dose of 10μg/105 cells, however thymocytes were not reactive. Stimulation effects on peritoneal macrophages from a guinea pig to incorporation of 14C-glucosamin were not exhibited on addition of 100μg of both peptidoglycans. These findings indicate that peptidoglycan of A.actinomycetemcomitans might eventually be responsible for destruction of periodontal tissue by host mediated activities. |
| Practice ： | Dentistry |
| Keywords ： | Peptidoglycan, Actinobacillus actinomycetemcomitans, Chemical structure, Immunomodulating activities |
