アブストラクト(56巻1号:神奈川歯学)

神奈川歯学

Japanese

Title : ヒトと飼いイヌにおける歯垢中の歯周病原菌の検出
Subtitle : 原著
Authors : 西山謙三, 佐々木悠, 稲葉啓太郎, 倉橋絢子, 浜田信城
Authors(kana) :
Organization : 神奈川歯科大学分子生物学講座口腔細菌学分野
Journal : 神奈川歯学
Volume : 56
Number : 1
Page : 10-18
Year/Month : 2021 / 6
Article : 原著
Publisher : 神奈川歯科大学学会
Abstract : 「緒言」 Porphyromonas gulae (P. gulae)は, イヌの歯肉溝から高頻度に検出される黒色色素産生性偏性嫌気性グラム陰性桿菌である. P. gulaeは, 2001年にさまざまな動物の歯肉溝に生息する新種として報告され, DNA-DNA相同性データと16SリボソームRNA(rRNA)遺伝子配列の分析により, P. gulaeがPorphyromonas gingivalis (P. gingivalis)とは異なる菌種であることが報告されている. イヌの約80%が4歳までにある程度の歯周病を示すと推定されており, 歯周病の重症度は年齢とともに悪化すると報告されている. また, 1mmを超えるアタッチメントロスの有病率は1歳のイヌで20%であり, 年齢にもよるが44~81%が4mm以上のプロービングポケットの深さを有することが報告されている. イヌの歯垢から分離されるPorphyromonas属の割合は, 5歳では2歳時に比較して約6倍になり, 疾患の重症度と相関することが報告されている.
Practice : 歯科学
Keywords : Porphyromonas gulae, Porphyromonas gingivalis, 歯周病原細菌, PCR

English

Title : Detection of periodontopathic bacteria in dental plaque of humans and their dogs
Subtitle :
Authors : Kenzo NISHIYAMA, Haruka SASAKI, Keitaro INABA, Ayako KURAHASHI, Nobushiro HAMADA
Authors(kana) :
Organization : Department of Oral Microbiology, Kanagawa Dental University
Journal : Kanagawa Shigaku
Volume : 56
Number : 1
Page : 10-18
Year/Month : 2021 / 6
Article : Original article
Publisher : Kanagawa Odontological Society
Abstract : [Abstract] Periodontitis is a chronic disease caused by Gram-negative, bacterial mixed infection. Porphyromonas gulae is a black-pigmented anaerobic bacteria associated with canine periodontitis. The properties of P. gulae fimbriae were reported to be similar to those of Porphyromonas gingivalis fimbriae. The purpose of the present study was to analyze the distribution of oral bacteria in dental plaque samples from humans and their dogs. We investigated whether P. gulae has the same ability to adhere to human cells as P. gingivalis. The possibility of transmission of P. gulae to the human oral cavity was investigated by PCR. In addition, PCR was used to analyze the distribution of P. gingivalis, P. gulae, Aggregatibacter actinomycetemcomitans, Campylobacter rectus, Fusobacterium nucleatum, Tannerella forsythia and Treponema denticola in dental plaque samples from dogs and their owners. P. gulae was able to adhere to human gingival fibroblasts as it was detected in dental plaque samples from humans living with dogs. On the other hand, human-derived bacteria were found in the oral cavity of dogs living with their owners. This study suggests that periodontopathic bacteria can be transmitted between humans and dogs.
Practice : Dentistry
Keywords : Porphyromonas gulae, Porphyromonas gingivalis, PCR