アブストラクト（18巻1号：神奈川歯学）

神奈川歯学

Japanese

Title　： 歯胚の生化学的研究 - 特にGlycosaminoglycansの変動について -
Subtitle　： 原著
Authors　： 中山芳夫
Authors（kana）　：
Organization　： 神奈川歯科大学小児歯科学教室
Journal　： 神奈川歯学
Volume　： 18
Number　： 1
Page　： 1-15
Year/Month　： 1983 / 6
Article　： 原著
Publisher　： 神奈川歯科大学学会
Abstract　： 「緒言」 結合組織における主要な非コラーゲン性物質であるGlycosaminoglycans(以下GAGsと略す)について検索することは, 歯胚形成過程における発育・成長の指標物質として極めて重要な事柄であるにもかかわらず, 殆んど知見が得られていない. 特に, 歯小嚢に関する報告は皆無であり, 近年, 歯根膜に関して榎本らの報告をみるのみである. また, 歯乳頭に関しても同様で, その報告は僅かであり, 萌出永久歯歯髄では, Linde, Murakawa, Orlowskyらの研究があるにすぎない. すなわち, ラット, ウサギおよびブタを用い, 分画法は異なるが, 50～80%がコンドロイチン硫酸で, 10～30%がヒアルロン酸, そして, わずかにデルマタン硫酸を含むという報告を行っている. これらは, いずれもProteoglycansの構成成分であり, 結合組織系細胞由来の有機質基質として, コラーゲンと共にその主要成分となっている.
Practice　： 歯科学
Keywords　：

English

Title　： Biochemical Study of Tooth Germ - Variation of GAGs During Tooth Germ Development -
Subtitle　：
Authors　： Yoshio NAKAYAMA
Authors（kana）　：
Organization　： Department of Pedodontics, Kanagawa Dental College
Journal　： Kanagawa Shigaku
Volume　： 18
Number　： 1
Page　： 1-15
Year/Month　： 1983 / 6
Article　： Original article
Publisher　： Kanagawa Odontological Society
Abstract　： Abstract : It is vitally important to study the biochemical processes of tooth germ development and eruption of the tooth, about which information remains scanty. The components of the organic matrix, especially glycoconjugates seem to relate to the calcification mechanism as well as to collagen. This study, therefore, was an attempt to focus on quantitative change in glycosaminoglycans (GAGs) in various stages of tooth germ development. Tooth germ collected from bovine mandibular bone were divided into three groups depending upon the degree of the root formation, i. e. - tooth germ which has little or slight formation of dentin is defined as stage 1, approximately 1/2 length of the root formed comprised as stage 2, those with almost full length of root formed were defined as stage 3, and teeth undergoing eruption were classified as stage 4. From each stage, dental sac and periodontal ligament were removed and dental papilla or pulp were dissected out, then each collected tissue was extracted with 0.16 and 1.00 M NaCl followed by precipitation with 25% saturated (NH4)2SO4. After desalting and lyophylization the precipitates were subjected to analysis for uronic acid to estimate the quantity of GAGs and determine the relative amount of hyaluronic acid, dermatan sulfate, and chondroitin -4 sulfate + chondroitin -5 sulfate using cellulose acetate electrophoresis according to Hata's method. It was clearly observed that the level of hyaluronic acid decreased rapidly during the three stages of tooth germ development most distinctively in the 1.0 M NaCI extraction of dental papilla or pulp, followed by remarkable increases of hyaluronic acid during tooth eruption as seen in the 0.16 M NaCI extraction of pulp. It was also found that the level of chondroitn -4 and -6 sulfate increased considerably according to the stage of tooth germ development and then rapidly decreased following tooth eruption in all fractions except the 0.16 M NaCI extraction of periodontal ligament. These results suggest the posibility of a mechanism for degradation of hyaluronic acid which forms the main polysaccharide chain of glycoconjugate and the increase of sulfated GAGs during tooth germ development. This mechanism may involve the release of divalent cations by the lysis of the protein core which links thc oligosaccharide chains. Furthermore, the increase in hyaluronic acid followed by decrease in sulfated GAGs might be related to the process of tooth eruption.
Practice　： Dentistry
Keywords　：