アブストラクト（18巻2号：神奈川歯学）

神奈川歯学

Japanese

Title　： LPS接種によるRat歯周組織の変化についての実験病理学的研究 - 特に歯槽骨吸収に与えるHeparinの影響について -
Subtitle　： 原著
Authors　： 清水洋子
Authors（kana）　：
Organization　： 神奈川歯科大学病理学教室
Journal　： 神奈川歯学
Volume　： 18
Number　： 2
Page　： 149-172
Year/Month　： 1983 / 9
Article　： 原著
Publisher　： 神奈川歯科大学学会
Abstract　： 「緒言」 現在, 内毒素endotoxin(lipopolysaccharide以下LPSと略)は歯周疾患の発症や増悪に関与する重要な因子の一つと考えられ, in vitroの実験系を中心として, このグラム陰性菌の細胞壁構成成分であるLPSが骨吸収作用を有するという事実が支持されている. また, 最近ではin vivoでその作用の確認を試みる動物実験も行われ, LPSが破骨細胞を賦活して破骨細胞性骨吸収を促進するという報告がなされているが, 一方ではin vitroの実験結果を支持するような骨吸収は生じないという報告もなされており, いまだ統一した見解が得られていない. さらに, GoldhaberはHeparinがin vitroで骨吸収を促進させるという報告を行い, 以来Heparinが生理的, 病理的, 骨吸収刺激に対するcofactor的役割をもつ可能性についても一部の研究者によって注目されていたが, 特にHausmannらはin vitroでHeparinがLPSによる骨吸収作用を助長(増強)することを報告している.
Practice　： 歯科学
Keywords　：

English

Title　： Experimental Pathological Study on Change in the Rat Periodontal Tissue Due to LPS Inoculation - with Special Reference to the Effect of Heparin on the Alveolar Bone Resorption -
Subtitle　：
Authors　： Yoko SHIMIZU
Authors（kana）　：
Organization　： Department of Pathology, Kanagawa Dental College
Journal　： Kanagawa Shigaku
Volume　： 18
Number　： 2
Page　： 149-172
Year/Month　： 1983 / 9
Article　： Original article
Publisher　： Kanagawa Odontological Society
Abstract　： Abstract : Time course of histopathological changes in periodontal tissue, mainly in alveolar bone induced by submucosal inoculation of lypopolysaccharides (LPS, E. coli : W. 0111, B4 ; Difco) and of heparin in maxillary gingiva were studied in rats. Specifically, osteoclastic bone resorption in the inoculated region and the relationship between the resorption and appearance potency of the tissue mast cells were investigated. The following results were obtained : 1. In the group treated with one or two times of inoculation of LPS, a few osteoclasts were observed in the margin of the bone edge ranging from the region on the periodontal side to the intraradicular septum two days after the inoculation. However, osteoclastic bone resorption was not observed in the dento-alveolar margin, as in the inoculated site within seven days after the inoculation. 2. In the group treated with four times of inoculation of LPS, the number of osteoclasts increased markedly. In addition, wavy findings of resorption were observed in the area ranging from the dento-alveolar margin to the region on the periodontal side and the intraradicular septum one to three days after the inoculation. Subsequently, the number of osteoclasts and the incidence of the wavy findings decreased five to seven days after the inoculation. 3. In the group inoculated with heparin plus LPS, a large number of osteoclasts and marked wavy findings of resorption were observed in the area ranging from the margin to the region on the periodontal side and the intraradicular septum on a day after the inoculation. This finding is different from that abserved after inoculation of LPS alone. Further, the number of osteoclasts associated with wavy findings of resorption was about twice as large as that in the group inoculated with heparin alone. 4. In both groups, inoculated with heparin plus LPS and with heparin alone, heparin caused dose-dependent increase in number of osteoclasts. 5. The histological characteristics of the inflammatory changes caused by inoculation of LPS or heparin plus LPS consist of a weak exudative mechanism in spite of neutrophilic infiltration. In this case, infiltration of lymphocytes and macrophages, appearance of mast cells and proliferation of the fibrous elements were observed in early inflammatory stage in contrast to the non-specific exudative inflmmation. 6. In the inflammatory lesion caused by inoculation of croton oil, as a control, within seven days after the inoculation mast cells were not observed due to the strong exudative mechanism. Further, no findings of osteoclastic bone resorption were observed. In contrast to that mentioned above, histological findings with a weak mechanism of infiltration were observed in the group inoculated with LPS, and the number of mast cells increased shortly, 10 minutes to six hours after the inoculation. This increase in number of mast cells was potentiated by heparin, and heparin enhanced the effect of LPS on osteoclastic bone resorption. 7. It is likely from overall results that in vivo osteoclastic bone resorption induced by LPS is mediated in part by heparin synthesized and stored in mast cells.
Practice　： Dentistry
Keywords　：