アブストラクト(19巻3号:神奈川歯学)

神奈川歯学

Japanese

Title : 実験的顎下腺腫瘍の病理発生とその増殖能に関する蛍光組織化学的研究
Subtitle : 原著
Authors : 片瀬秀士
Authors(kana) :
Organization : 神奈川歯科大学病理学教室
Journal : 神奈川歯学
Volume : 19
Number : 3
Page : 169-198
Year/Month : 1984 / 12
Article : 原著
Publisher : 神奈川歯科大学学会
Abstract : 「緒言」齧歯類の唾液腺に発生する実験腫瘍は癌原性炭化水素の出現後, 比較的高率に発生させることが出来るようになり, 光顕的, 電顕的観察を中心とした形態面での研究が数多く報告されている. また, 癌原性炭化水素によって発生する齧歯類の顎下腺腫瘍はその多くが扁平上皮癌であり, この癌腫発生過程に類表皮嚢胞の形成が多くの腫瘍発生実験で観察されている. しかし, 現在まで形態学的な面からみた腫瘍発生の報告は数多くなされているものの, その腫瘍発生過程或いは発生腫瘍についての核DNA量の面からみた検索はなされていない. 一方, 近年顕微分光測光法による組織細胞レベルでのFeulgen核DNA量の測定 (定量) が可能となり, 種々の悪性腫瘍細胞の染色体異常が染色モードを反映して核DNA量の変異として出現することが明らかとなった. そこで種々のヒト腫瘍における悪性度の客観的指標として, 従来から行われている腫瘍の病理組織学的診断に加え, Feulgen核DNA量の測定が試みられている.
Practice : 歯科学
Keywords :

English

Title : Fluoresence Histochemical Study of the Pathogenesis of Experimental Tumors of the Submandibular Gland and their Growth
Subtitle :
Authors : Hideji KATASE
Authors(kana) :
Organization : Department of Pathology, Kanagawa Dental College
Journal : Kanagawa Shigaku
Volume : 19
Number : 3
Page : 169-198
Year/Month : 1984 / 12
Article : Original article
Publisher : Kanagawa Odontological Society
Abstract : A DMBA pellet (9,10-dimethyl-1, 2-benzanthracene) was inserted into the submandibular glands of male Fischer rats to investigate the process in which the squamous metaplasia occurring in tumorigenesis progresses to squamous cell carcinoma. A correlation was made between tumor tissue type and the amount of nuclear DNA, as determined by Feulgen's method (Experiment 1). In addition, a portion of the tumor tissue was autotransplanted into areas under the abdominal fascia to examine the correlation between the amount of nuclear DNA in the autograft and its growth in the transplanted focus (Experiment 2). Experiment 1 1) Squamous metaplasia of the residual excretory or repair ducts began two weeks after the insertion of the pellet, and after five weeks a keratinized cyst was formed around the pellet, resulting in an atypical appearance of the metaplastic epithelium lining the back of the cyst wall. In all groups, 9, 10, 11, 13 and l5 weeks after pellet insertion, about 40% of the rats showed the metaplastic epithelial growth of the cyst wall. Thirteen weeks after pellet insertion, this growth was marked, and dyskeratosis and an unclear basement membrane were observed. 2) During tumorigenesis, the amount of nuclear DNA showed a wide, double or multipeak histogram pattern extending to the octaploid area from five weeks after pellet insertion, nearly coinciding with the development of the atypical backing epithelium. In 33-60% of rats between seven and 15 weeks after pellet insertion, a shift of the major mode to the triploid area, an unclear major mode, or the appearance of octaploids, were noted. 3) Of 20 rats, 24 weeks after the insertion of the DMBA pellet, 11 showed tumors of the submandibular gland (keratinized squamous cell carcinoma, 9; carcinosarcoma, 2). In five (45%) of the 11 rats, the amount of nuclear DNA in the tumor showed a malignant histogram pattern exhibiting many peaks, disappearance of the major mode, or the appearance of octaploids Experiment 2 4) Tumor of the submandibular gland occurred in 20 of 45 rats between 21 and 30 weeks after the insertion of a DMBA pellet (44.4%; 19 cases of keratinized squamous cell carcinoma, one case of sarcomatoid tumor). 5) Autotransplantation in areas under the abdominal fascia resulted in the growth of an autograft in six of 14 rats (42.8%; one case of keratinized squamous cell carcinoma, two of cystic tumor, one of squamous cell carcinoma, two of solid proliferative tumor, and one of sarcomatoid tumor). The histopathological findings of the transplanted focus showed tumor tissues similar to those in the primary focus, i. e., the autograft. 6) Analysis of the correlation between the tumor growth in the autotransplanted focus and the amount of nuclear DNA in the tumor cells in the graft revealed a malignant histogram pattern (shift of the major mode from the triploid to the octaploid area and the appearancc of high polyploidy) in rats showing growth of the transplanted focus. In contrast, eight rats showingno growth of the transplanted focus had convergent single-peak histogram patterns with the major mode in the diploid area. In addition, the PI (proliferative index), in terms of the percentage of tumor cells corresponding to DNA synthesis stage and mitotic stage, was as high as 52%-93.4% in rats showing growth of the transplanted focus, whereas it was as low as 19%-37.9% in rats without such growth. Thus, an obvious correlation was found between the growth of the autotransplanted focus and the amount of nuclcar DNA of the transplanted tumor cells.
Practice : Dentistry
Keywords :