アブストラクト(22巻3号:神奈川歯学)

神奈川歯学

Japanese

Title : SomatostatinのRadioimmunoassayによる基礎的検討
Subtitle : 原著
Authors : 木村友七, 伊東利, 石井寸実代, 岩宮万里子, 林比呂志, 早川千春
Authors(kana) :
Organization : 神奈川歯科大学臨床病理学教室, 中央検査科
Journal : 神奈川歯学
Volume : 22
Number : 3
Page : 519-524
Year/Month : 1987 / 12
Article : 原著
Publisher : 神奈川歯科大学学会
Abstract : 「はじめに」Somatostatin(SST)は, ヒト成長ホルモン(GH)分泌を抑制する以外に, 下垂体前葉ホルモン(TRH)による甲状腺刺激ホルモン(TSH)分泌を抑制する. Brazeauら(1973)により, ヒツジ視床下部から分離その構造を決定してから, 有意義な報告と作用が認められている. ArimuraらによりSSTのradioimmunoassay(RIA)法による測定がされてから, glucocorticoid inhibiting factor(GIF)の動態に関する薬理・生化・分泌生理・組織・臨床治療学的に種々の研究報告がされている. 生理的関与, 疾患との関連, その病態と検索の意義やその価値を解明するために, SSTの生体内動態における基礎的検索を行い, 若干の知見を得たので報告する. 「検索資料, 測定方法」「1. 検索健常者の選出, 前準備, 採血条件」1)成人健常者の選出:健康状態の把握は, 作製した問診票を参考に自・他覚的に健康な本学学部5年生(男性18名, 23-25歳), 女子短大生(81名, 18-20歳)の合計99名である.
Practice : 歯科学
Keywords : Somatostatin, Blood, RIA法

English

Title : The Development of a Radioimmunoassay for Somatostatin
Subtitle :
Authors : Tomoshichi KIMURA, Satoshi ITO, Sumiyo ISHII, Mariko IWAMIYA, Hiroshi HAYASHI, Chiharu HAYAKAWA
Authors(kana) :
Organization : Department of Clinical Pathology and Central Laboratories, Kanagawa Dental College
Journal : Kanagawa Shigaku
Volume : 22
Number : 3
Page : 519-524
Year/Month : 1987 / 12
Article : Original article
Publisher : Kanagawa Odontological Society
Abstract : [Abstract]: SST (Somatostatin) measurement was carried out on human blood after dividing into test groups 1 and 2. The following results were obtained. 1. Healthy male students at this institution as well as healthy female students from the Women's Junior College of Hygiene for a total of 99 people between 18-25 were used as test subjects. Use was made of the Aceton-ether method and measurements were performed with the RIA method. 2. Plasma separation and examination for group 1 was done at room temperature by centrifugation at 3,000 rpm for 10 minutes. The dried plasma obtained with the thermostat had a mean value of 42.28, an SD of 17.34 and a normal value of 24.9-59.62 pg/ml. 3. Plasma separation for group 2 was done with a Himac Centrifuge Separator at 4℃ at a rate of 3,000 rpm for 10 minutes. The dried plasma was measured by means of the RIA method using SST from the Immuno Corporation to give the following results. (1) A total of 81 test subjects, both male and female, showed a mean value of 21.7, an SD of 9.5 and a normal value of 12.2-31.2 pg/ml. (2) A total of 18 male test subjects showed a mean value of 27.1, an SD of 12.8 and a normal value of 14.3-39.9 pg/ml. (3) A total of 63 females test subjects showed a mean value of 20.2, an SD of 7.7 and a mormal value of 12.5-27.9 pg/ml. 4. Group 1 showed higher values than group 2. In addition, the males in group 2 showed a tendency toward higher values than the females.
Practice : Dentistry
Keywords : Somatostatin, Blood