アブストラクト(23巻2号:神奈川歯学)

神奈川歯学

Japanese

Title : ヒト歯根膜線維芽細胞の生化学的研究 - 機械的外力による細胞の応答性について -
Subtitle : 原著
Authors : 岸秀晃, 斎藤滋
Authors(kana) :
Organization : 神奈川歯科大学口腔生化学教室
Journal : 神奈川歯学
Volume : 23
Number : 2
Page : 223-234
Year/Month : 1988 / 9
Article : 原著
Publisher : 神奈川歯科大学学会
Abstract : 「緒言」歯周組織は常に咬合力を受け, 特に歯根膜は歯と歯槽骨と連結する強靱な結合組織である. 咬合力の変化あるいは矯正力などによって明らかに歯周組織の組織改造が起こる. そのメカニズムを明らかにすることは大変意義のあることである. そこで機械的外力を培養細胞に加え, その応答機序を細胞生物学的に検索した. すなわち, 歯根膜由来線維芽細胞(HPLF)はすでに, 斎藤らによりヒト歯根膜のexplant法を用いて培養が可能になり, 細胞の種々の特徴からosteoblastic fibroblastと提唱された. すなわち, HPLFは高いALPaseを示し, 1, 25(OH)2 D3によってALPase活性は上昇し, しかもALPaseは骨タイプであることが明らかにされた. 一方, 細胞に対する機械的外力として, 斎藤は1) tension and compression, 2) gravity, 3) atomspheric, 4) thermodynamic stress (heat shock) さらに5) electromagnetic field に分類している.
Practice : 歯科学
Keywords : ヒト歯根膜線維芽細胞, 機械的外力, アルカリフォスファターゼ

English

Title : Biochemical Study of Human Periodontal Ligament Fibroblasts - Cellular Responses by a Mechanical Stress -
Subtitle : Original article
Authors : Hideaki KISHI, Shigeru Saito
Authors(kana) :
Organization : Department of Oral Biochemistry, Kanagawa Dental College
Journal : Kanagawa Shigaku
Volume : 23
Number : 2
Page : 223-234
Year/Month : 1988 / 9
Article : Original article
Publisher : Kanagawa Odontological Society
Abstract : Abstract : The effect of mechanical stress on cell growth and differentiation is the subject of increasing interest and research. In this study, human periodontal ligament fibroblasts (HPLF), stressed by mechanical stretching, secrete substances which act factors inhibititing cell growth and stimulating alkaline phosphatase (ALPase) activity in both HPLF and human alveolar bone cells (HABC). HPLF and HABC were obtained by the explantation methold described by Saito et al (IADR, 1987). HPLF were cultured in a Petriperm dish whose bottom is made of a flexible plastic membrane which can be stretched by application of a weight, as described in the method of Hasegawa et al (1985). Confluent cells, inoculated at a density of 2×104 cells/cm2, were alternately "stretched" in this manner, then "relaxed" for periods of 60 minutes each, for a total of 12 hours. Mediums of stretched (S-CM) and non-stretched (NS-CM) cells were then collected and centrifuged. Mediums of 20% S-CM and NS-CM were added to HPLF, HGF, HABC and UMR and cultured for 9 days. Protein content of cell extracts was determined as described by Bradford (1976). ALPase activity was assayed at 37°C by the method described by Puzas and Brand (1985). After 24hrs incubation, HPLF, HABC and UMR inoculated were incubated with conditioned mediums and 3H-thymidine (2μCi/ml) for 5hrs. And, HPLF, HABC and UMR were incubated with 50% conditioned medium and then observed by a phase contrast microscope. The protein content of cell layers of HPLF, HGF and HABC were more significantly decreased by S-CM than by NS-CM. ALPase activity of HPLF was increased 1.71 fold by NS-CM and 2.63 fold by S-CM. Neither the protein content nor the ALPase activity of UMR was affected by either NS-CM or S-CM. The incorporation of 3H-thymidine into DNA incubated with S-CM was remarkably inhibited. On the other hand, S-CM stimulated cell spreading after cell inoculation and adhesion. Therefore : mechanical stress may regulate cell metabolism of HPLF, stimulating secretion of a negative cellular autocrine growth factor, which might itself control cellular response.
Practice : Dentistry
Keywords :