アブストラクト（23巻4号：神奈川歯学）

神奈川歯学

Japanese

Title　： 根尖病変成立に関する細菌学的免疫学的研究 - Bacteroides buccae菌体成分の免疫生物活性と根尖病変における局在 -
Subtitle　： 原著
Authors　： 谷信之1),2), 長田保1), 梅本俊夫2)
Authors（kana）　：
Organization　： 1)神奈川歯科大学歯科保存学教室第三講座, 2)神奈川歯科大学口腔細菌学教室
Journal　： 神奈川歯学
Volume　： 23
Number　： 4
Page　： 451-468
Year/Month　： 1989 / 3
Article　： 原著
Publisher　： 神奈川歯科大学学会
Abstract　： 「緒言」根尖病変は, 根管内細菌に由来する菌体成分や産生物, あるいは歯髄由来の自己融解組織などの刺激に対する免疫応答により惹起されると考えられている. 従って, 感染根管内細菌は, 根尖病変の発現に関しても重要な因子であると考えられており, 多くの研究者が根管内細菌叢の解析を行ってきた. 近年, 嫌気性菌の培養および分離技術の改良に伴って根管内細菌叢の全容が嫌気性菌を中心に急速に解明されてきており, 根尖病変を有する根管内からは, 高頻度に偏性嫌気性菌が分離されること, また臨床症状の増悪に伴ってこの傾向は顕著になることが報告されている. 菌種としては, 特にBacteroides, Peptostreptococcus, EubacteriumおよびVeillonellaが高頻度に分離されることが共通して報告されている. これらの根管内細菌は, 種々の酵素や毒素を産生し直接的に組織を傷害すると同時に生体における免疫応答を引き起こし, 間接的に根尖歯周組織を傷害する可能性が示唆されている.
Practice　： 歯科学
Keywords　： 根尖病変, 根管内細菌, 免疫生物活性

English

Title　： Bacteriological and Immunological Studies on the Mechanism of Development of Periapical Lesion - Immunobiologigcal Activities and Localization in Periapical Lesion of the Cellular Components from Bacteroides buccae -
Subtitle　： Original article
Authors　： Nobuyuki TANI1),2), Tamotsu Osada1), Toshio Umemoto2)
Authors（kana）　：
Organization　： 1)Department of Conservative Dentistry, Kanagawa Dental College, 2)Department of Oral Microbiology Kanagawa Dental College
Journal　： Kanagawa Shigaku
Volume　： 23
Number　： 4
Page　： 451-468
Year/Month　： 1989 / 3
Article　： Original article
Publisher　： Kanagawa Odontological Society
Abstract　： [Abstract]: It has been strongly suggested that the periapical lesion develop as a result of immunopathological response to continuous antigenic stimulation. Bacteria from root canal systems might be most important pathogenesis capable of inducing immunological reactions in periapical tissue. The purpose of this study, therefore, was clarify the immunological potentials of Bacteroides buccae (B.buccae) which was frequently isolated from root canals with chronic periapical lesion. Biological activities of B.buccae cellular components, such as lipopolysaccharides (LPS) and cellular protein were investigated on the enhancement of monocytes migration induction of inter-leukin 1 (IL-1) production, mitogenicity and polyclonal B cell activation. The localization of immunocompetent cells and B.buccae in human chronic periapical lesions were examined by biotin-avidin-horseradish peroxidase method, and peroxidase antiperoxidase methods using monoclonal antibodies. Following result were obtained. 1. On the lymulus lysafe clotting activity and Shwarzman activity of B.buccae LPS were about half of Salmonella typhimurium (S.typhimurium). 2. Both LPS and 38K protein preparations from B.buccae enhanced the activity of human peripheral monocytes migration and induced IL-1 production. 3. It was found that mitogenicity of LPS from B.buccae on splenocytes of BALB/c and BALB/c nu/nu mice was weaker than that of 38K protein, however mitogenicity on thymocytes were not shown in both preparations. 4. The polyclonal B cell activation on splenocytes of BALB/c nu/nu mice by B.buccae were remarkably induced by 38K protein, but LPS showed less activity elicit than 38K protein. 5. It might be suggested that the both LPS and 38K protein of B.buccae may depend on the activities of macrophage and lymphocytes. 6. Antigenic substance of B.buccae were found most commonly engulfed materials within macrophage and intercellular space in connective tissue. 7. Dense accumulation of T and B lymphocytes were observed gathering around the phagocytic macrophage (foam cell), the number of B lymphocytes around the macrophage was greater than that of T lymphocytes. 8. These findings indicated that both LPS and 38K protein from B.buccae have a wide regulation function of immunobiologic responses. Therefore, it was suggested that both LPS and 38K protein from B.buccae may play significant roles in the pathogenesis of periapical lesion.
Practice　： Dentistry
Keywords　：