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アブストラクト（24巻4号：神奈川歯学）
Japanese
| Title ： | ヒト歯根膜培養細胞における重金属の影響に関する研究 -特にPbおよびCdについて- |
|---|---|
| Subtitle ： | 原著 |
| Authors ： | 葉山淑人*1, 檜垣旺夫*1, 斎藤滋*2 |
| Authors（kana） ： | |
| Organization ： | *1神奈川歯科大学小児歯科学教室, *2神奈川歯科大学口腔生化学教室 |
| Journal ： | 神奈川歯学 |
| Volume ： | 24 |
| Number ： | 4 |
| Page ： | 671-691 |
| Year/Month ： | 1990 / 3 |
| Article ： | 原著 |
| Publisher ： | 神奈川歯科大学学会 |
| Abstract ： | 「緒言」 重金属は生体に対して様々な影響を及ぼすといわれ, 特に成長期においてその影響は大きいと考えられる. したがって, 乳歯萌出から永久歯列完成に至るまでの長期の口腔の成長変化を育成・管理するためには, 歯および歯周組織と生体に対し有害と考えられる重金属との関連性について解明することは重要である. 現在まで歯の発育に関する研究は多岐にわたって行われており, 特に交換期乳歯を使用した歯質中の微量元素の測定に関する研究などの報告は数多い. しかしこれらの研究は, 過去の発育環境の検討には非常に適している反面, その形成過程での微細な動態を知ることはできない. 石灰化への重金属の影響については動物を用いたGinnら, Woltgensらなどの報告があるが, ヒト正常組織あるいは正常細胞による報告はほとんどない. ヒト歯周組織はSomermanらにより高い石灰化能を有していることが報告されている. |
| Practice ： | 歯科学 |
| Keywords ： | ヒト歯根膜, 鉛, カドミウム |
English
| Title ： | Effect of Heavy Metal Ions on the Cells Derived from Human Periodontal Ligament -Effects of Pb and Cd- |
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| Subtitle ： | |
| Authors ： | Yoshito HAYAMA*1,*2, Morio Higaki*1, Shigeru Saito*2 |
| Authors（kana） ： | |
| Organization ： | *1Department of Pediatric dentistry, Kanagawa Dental College, *2Department of Oral Biochemistry, Kanagawa Dental College |
| Journal ： | Kanagawa Shigaku |
| Volume ： | 24 |
| Number ： | 4 |
| Page ： | 671-691 |
| Year/Month ： | 1990 / 3 |
| Article ： | Original article |
| Publisher ： | Kanagawa Odontological Society |
| Abstract ： | [Abstract]: It has been thought that the incorporation of Pb and Cd into human body, especially calcified tissues including the tooth, has altered the physiological processes of development. But, the biochemical events that initiate this process remain quite unknown. The present study attempt to explore the effect of Pb and Cd on human periodontal ligament fibroblast-like cells of the permanent tooth (HPLF) and the deciduous tooth (HPLF-Y) with respect to the cell growth, ALPase activity and incorporation of 14C-amino acids. HPLF and HPLF-Y migrated from a explant and subcultured according to the previously described method of Saito were inoculated 1.25 × 104 cells/cm2 in D-MEM supplemented with 2 mg/ml FCSP, 50 μg/ml ascorbic acid and antibiotics. After 24hrs, HPLF and HPLF-Y were treated every two days for 10 days with 0 - 200 μM Pb or 0 - 10 μM Cd. Protein contents, DNA contents and ALPase activity were determined by Bio-Rad protein assay, deaminobenzoic acid assay and p-nitrophenylphosphate (pH 10.15) assay respectively. At 6 days HPLF were incubated with 3H-thymidine (TdR 2.0μ Ci/well) and then the incorporation of 3H-TdR into cold TCA precipitates was assayed by a liquid scintillation counter. And also, at 6 days HPLF and HPLF-Y were incubated with 14C-amino acids (2.0μ Ci/60mm dish) for 24 hrs. The cell layers labeled with 14C were extractd with 15 mM Tris-HCl buffer containing 7 M urea (pH 7.4) and applied to the gel permeation chromatography of HPLC system to separate the fractions according to molecular weight. The HPLF and HPLF-Y incubated with Pb and Cd were morphologically identical. Pb stimulated the protein contents of extracellular matrix of HPLF, but not HPLF-Y. Cd inhibited the protein contents of cell layers of HPLF and HPLF-Y. With increasing concentrations of Pb and Cd, the incorporation of 3H-TdR into HPLF was inhibited. On the other hand, Cd stimulated the ALPase activity per DNA content since the ALPase activity of HPLF and HPLF-Y was decreased by the addition of Pb. The distribution of 14C-labeled protein according to molecular weight did not alter the chromatographic pattern of HPLF incubated with Pb and Cd. But, that of HPLF-Y incubated with Pb was relatively shifted to low molecular size. Therefore, these responser concludeed that HPLF were not completely identical with HPLF-Y. Pb and Cd not only had a toxic effect on cell growth, but also they may regulate the metatabolic alteration. |
| Practice ： | Dentistry |
| Keywords ： |
