アブストラクト（24巻4号：神奈川歯学）

神奈川歯学

Japanese

Title　： ヒト歯根膜細胞(HPLF)におけるパルス電磁場の影響 -細胞内カルシウム[Ca2+]iの変化について-
Subtitle　： 原著
Authors　： 佐竹司, 安伸浩, 抱井淑郎, 川村太助, 佐藤透, 中野完, 網野省三, 石渡庸介*, 斎藤滋, 堀俊雄*
Authors（kana）　：
Organization　： 神奈川歯科大学口腔生化学教室, *神奈川歯科大学保存学教室第2講座
Journal　： 神奈川歯学
Volume　： 24
Number　： 4
Page　： 735-742
Year/Month　： 1990 / 3
Article　： 原著
Publisher　： 神奈川歯科大学学会
Abstract　： 「緒言」 歯根膜由来線維芽細胞(HPLF)は, すでに斎藤らにより, ヒト歯根膜のexplant法を用いて培養が可能となった. HPLFは高いAlkaline phosphatases (ALPase)活性を有し, 1,25 (OH)2 D3により, 活性の上昇が認められる. 歯根膜は, 歯槽骨と歯に対して緩衝的な役割をし, 常に咬合力すなわち機械的外力が加わっている. そのため, 歯周組織に機械的外力を添加した際に生じる現象を生化学的に検索する事は, 臨床, 基礎歯科医学両面に対して重要といえる. 岸は, Hasegawaらの方法に従い歯根膜細胞に機械的刺激を加えたことによるALPase活性の上昇, 形態学的な変化を報告している. また, 骨に機械的外力を加えた場合電位差が生まれることが現在広く知られているが, これは骨組織のみならず, 腱, 関節軟骨, そして歯根膜などにも共通して観察される現象である.
Practice　： 歯科学
Keywords　： PEMF, human periodontal ligament, 細胞内カルシウム〔Ca+2〕i, EGF, TPA

English

Title　： Effect of Pulsed Electromagnetc Fields (PEMF) on human periodontal ligament in vitro -Alterations of Intracellular Ca2+-
Subtitle　：
Authors　： Tsukasa SATAKE, Nobuhiro YASU, Yoshio KAKAI, Taisuke KAWAMURA, Tohru SATO, Tamotsu NAKANO, Shozo AMINO, Yosuke ISHIWATA*, Shigeru SAITO, Toshio Hori*
Authors（kana）　：
Organization　： Department of Oral Biochemistry, Kanagawa Dental College, *Department of Periodontology, Kanagawa Dental College
Journal　： Kanagawa Shigaku
Volume　： 24
Number　： 4
Page　： 735-742
Year/Month　： 1990 / 3
Article　： Original article
Publisher　： Kanagawa Odontological Society
Abstract　： [Abstract]: The concept of orthodontic tooth movement is based on the hypothesis that teeth move as a result of the biological response of periodontal tissues to the mechanical forces applied. There is a widely held hypothesis that mechanical stress generates an electrical signal which sets in motion the subsequent events, as in bone exposed to mechanical forces electrical currents are produced affect bone growth and remodeling. This implies a transduction mechanism which translates the electrical signal into a biochemical message, recognizable by the cellular machine. This study is aimed at the identification of the message and the investigation of its control. In fact, the effect of Pulsed Electromagnetic Fields (PEMF) on the intracellular second messenger, cytoplasmic Ca2+ in Human Periodontal Ligament Fibroblasts (HPLF) was investigated. The resting intracellular ionized calcium concentration ([Ca+2] i) of HPLF cells was 232.7 +- 25.0 nM, and with PEMF [Ca2+] i increased from 12 hrs to 499.0 +- 115.5 nM up to 12 hrs, then reached to a steady level through 24 hrs. The PEMF were also found to decrease the responses towards epidermal growth factor (EGF) and serum, when the degree of response was based on the intracellular Ca2+ transient. These effects of PEMF were mimicked by 12-O-tetradecanoyl phorbor 13-acetate (TPA), a potent activator of protein kinase C. Some reports have suggested that fibroblasts of the periodontal ligament contain high alkaline phosphatase (ALPase) activity as much as osteoblast. Since similar results concerning the [Ca2+] i were obtained in osteoblast (OB)-like cells, this experiment also supports the hypothesis that fibroblasts of periodontal ligament have osteoblastic features.
Practice　： Dentistry
Keywords　： PEMF, human periodontal ligament, EGF, TPA