- HOME
- > 一般の方
- > バックナンバー：神奈川歯学
- > 25巻3号
- > アブストラクト
アブストラクト（25巻3号：神奈川歯学）
Japanese
| Title ： | Capnocytophaga species細胞壁ペプチドグリカンの化学構造と免疫生物活性 |
|---|---|
| Subtitle ： | 原著 |
| Authors ： | 花形哲夫, 梅本俊夫 |
| Authors（kana） ： | |
| Organization ： | 神奈川歯科大学口腔細菌学教室 |
| Journal ： | 神奈川歯学 |
| Volume ： | 25 |
| Number ： | 3 |
| Page ： | 316-326 |
| Year/Month ： | 1990 / 12 |
| Article ： | 原著 |
| Publisher ： | 神奈川歯科大学学会 |
| Abstract ： | 「緒言」 歯周病の病因に関して, 各病型毎に, それぞれ特異的な細菌あるいは細菌群が関与していることが示唆されており, Socranskyはこれらの菌群を歯周病原細菌(periodontopathic bacteria)と呼び, それぞれの病型と特異的な関係にあると述べている. Newman (1976)らは, 局所型若年性歯周炎(localized juvenile periodontitis)のポケット内細菌叢の検索を行い, Actinobacillus actinomycetemcomitansとCapnocytophaga speciesが高率に検出されることを明らかにした. Capnocytophaga属は発育にCO2を要求するグラム陰性の彎曲細菌で, 糖を発酵的に分解し, 滑走運動を示す. 本菌属はDNAの相同性に基づいてC. sputigena, C. gingivalisおよびC. ochraceaの3種に分類されており, IrvingらはC. sputigenaがラットへの単一感染において歯根膜破壊を惹起する強い病原性を示すことを明らかにしており, Stevensらは, C. sputigenaのエンドトキシンに関して, 発熱性, ニワトリおよびマウス胎児に対する致死作用, 白血球毒性, マイトゲン活性, マクロファージ活性化作用およびヒト由来線維芽細胞に対する増殖阻止活性など各種の生物活性について検討し, その病原的意義を示唆している. |
| Practice ： | 歯科学 |
| Keywords ： | Peptidoglycan, Capnocytophaga species, Chemical structure, Immunobiological activity |
English
| Title ： | Chemical Structure and Immunobiological Activities of Peptidoglycan Isolated from Capnocytophaga species |
|---|---|
| Subtitle ： | |
| Authors ： | Tetsuo HANAGATA, Toshio Umemoto |
| Authors（kana） ： | |
| Organization ： | Department of Oral Microbiology, Kanagawa Dental College |
| Journal ： | Kanagawa Shigaku |
| Volume ： | 25 |
| Number ： | 3 |
| Page ： | 316-326 |
| Year/Month ： | 1990 / 12 |
| Article ： | Original article |
| Publisher ： | Kanagawa Odontological Society |
| Abstract ： | [Abstract]: The chemical structure and immunobiological activities of the cell wall peptidoglycan isolated from Capnocytophaga species was investigated. Peptidoglycan was isolated from Capnocytophaga species strain SE2-2 by boiling in 4% soduim dodecyl sulfate and by digestion with pronase, trypsin and α-amylase. Analysis of amino acids and amino sugars of the peptidogylcan revealed that glucosamine, muramic acid, D-glutamic acid, alanine, and diaminopimelic acid (A2pm) were the principal components. Serine and glycine were not found. Dinitrophenylation method revealed that about half of A2pm residue had a free amino group, and analysis by hydrazinolysis showed that a small part of alanine and A2pm located at the C-terminal. The above results indicate that one of the amino groups of A2pm residue at one strand of the stem peptide subunit cross-linked to the carboxyl group of alanine of the neighboring strand. It was thus revealed that the peptidoglycan of Capnocytophaga species belonged to the Alγ type of the classification by Schleifer and Kandler. Peptidoglycan isolated from Capnocytophaga species strain SE2-2 was found to be definitely adjuvant-active in induction of delayed type hypersensitivity against ovalbumin when administered to guinea pigs as water-in-oil emulsion and in stimulation of increase serum antibody levels. Regarding mitogenicity on splenocytes of BALB/c and BALB/c nu/nu mice, peptidoglycan from Capnocytophaga species was markedly enhanced the uptake [3H] thymidine in dose of 10μg/105 cells, however thymocytes were not reactive. Stimulation effects on peritoneal macrophages from a guinea pig to incorporation of 14C-glucosamin was exhibited by addition of 100μg of this peptidoglycan. These findings indicate that peptidoglycan of Capnocytophaga species might eventually be responsible for destruction of periodontal tissue by host mediated activities. |
| Practice ： | Dentistry |
| Keywords ： | Peptidoglycan, Capnocytophaga species, Chemical structure, Immunobiological activity |
