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アブストラクト（25巻3号：神奈川歯学）
Japanese
| Title ： | ヒト歯根膜線維芽細胞(HPLF)におけるPhosphotyrosine Phosphatase活性について |
|---|---|
| Subtitle ： | 原著 |
| Authors ： | 石渡庸介*1,*2, 堀俊雄*1, 斎藤滋*2 |
| Authors（kana） ： | |
| Organization ： | *1神奈川歯科大学保存学教室第二講座, *2神奈川歯科大学口腔生化学教室 |
| Journal ： | 神奈川歯学 |
| Volume ： | 25 |
| Number ： | 3 |
| Page ： | 355-368 |
| Year/Month ： | 1990 / 12 |
| Article ： | 原著 |
| Publisher ： | 神奈川歯科大学学会 |
| Abstract ： | 「緒言」 歯周組織の歯根膜と歯槽骨は常時, 間欠的な咬合力を受けており, しかも歯根膜は発生学的に歯小嚢から由来し, 同じ由来組織である歯槽骨とセメント質の間に位置する非石灰化の結合組織である. 一方, 近年, 斎藤らは, ヒト歯根膜由来の線維芽細胞(Human Periodontal Ligament Fibroblast : HPLF)の培養系を確立し, このHPLFの特徴は高いアルカリフォスファターゼ(ALPase)活性をもつこと, p-nitrophenylphosphateを基質とするKm値が骨芽細胞と近似の0.43mMであった. また, dexamethasone (Dex)とCa2+ regulatory hormoneである1,25 (OH)2 D3 (D3)により, ALPase活性が上昇し, このALPaseは熱に不安定で, L-homoarginineにより活性が阻害される. |
| Practice ： | 歯科学 |
| Keywords ： | HPLF, PTPase, 細胞接着因子 |
English
| Title ： | Phosphotyrosine Phosphatase Activity of Human Periodontal Ligament Fibroblasts (HPLF) |
|---|---|
| Subtitle ： | |
| Authors ： | Yousuke ISHIWATA*1,*2, Toshio Hori*1, Shigeru Saito*2 |
| Authors（kana） ： | |
| Organization ： | *1Department of Periodontology, Kanagawa Dental College, *2Department of Oral Biochemistry, Kanagawa Dental College |
| Journal ： | Kanagawa Shigaku |
| Volume ： | 25 |
| Number ： | 3 |
| Page ： | 355-368 |
| Year/Month ： | 1990 / 12 |
| Article ： | Original article |
| Publisher ： | Kanagawa Odontological Society |
| Abstract ： | [Abstract]: The periodontal ligament is a non-calcified connective tissue involved in the remodeling processes associated with mechanical stresses such as occlusion and mastication. This study was attempted to explore the characterization of phosphotyrosine phosphatase (PTPase) on the cell attachment and spreading of human periodontal ligament fibroblasts (HPLF). HPLF obtained by the explantation of human periodontal ligaments were cultured with vitamin D3 (D3) and dexamethasone (Dex) for 12 days. At 7 day culture, PTPase and ALPase activities were assayed with the substrates of phosphotyrosine and p-nitrophenylphosphate, respectively. Although ALPase activity of HPLF was dramatically increased by D3 and Dex, PTPase activity was not altered. After the quiescent HPLF were cultured with serum-free MCDB 107, the conditioned medium of HPLF (HPLF-CM) was coated on a hydrophobic dish. And then, HPLF were inoculated and cultured with 32Pi on the dish. PTPase activity of HPLF was decreased compared with control medium, however, the incorporation of 32Pi added in the medium was increased 2-fold. Therefore, HPLF possess the PTPase which may dephosphorylate a phosphotyrosine of phosphoproteins, which can regulate the cell proliferation. The cell attachment and spreading factors contained in HPLF-CM might alter the processes of phosphorylation and dephosphorylation of phosphoproteins. |
| Practice ： | Dentistry |
| Keywords ： | HPLF, PTPase |
