アブストラクト（27巻2号：神奈川歯学）

神奈川歯学

Japanese

Title　： ヒト歯根膜線維芽細胞由来の細胞接着・伸展因子の特異性
Subtitle　： 原著
Authors　： 網野省三, 川瀬俊夫, 斎藤滋
Authors（kana）　：
Organization　： 神奈川歯科大学口腔生化学教室
Journal　： 神奈川歯学
Volume　： 27
Number　： 2
Page　： 179-192
Year/Month　： 1992 / 9
Article　： 原著
Publisher　： 神奈川歯科大学学会
Abstract　： 「緒言」歯根膜は歯槽骨とセメント質の石灰化組織の間に位置した非石灰化の結合組織で, 間欠的な咬合力を受けている. しかし, 歯根膜は発生学的に歯槽骨とセメント質と同様に歯小嚢由来であり, 間葉系細胞である線維芽細胞のprogenitor cellsばかりでなく, 歯槽骨側の骨芽細胞とセメント質のセメント芽細胞のprogenitor cellsも含むとの報告がある. この様に, 歯根膜を構成する細胞の特微を明らかにすることで, 始めて, 歯周疾患での再生および再付着の機序が明らかになると思われる. そこで, このような関点から近年, Kawase, T.et al. Saito, S.et alにより, ヒト歯根膜組織からexplant法を用いてヒト歯根膜由来線維芽細胞(HPLF)の培養系が確立された. さらにKawase, T.et al.はHPLFに骨/腎臓/肝型のALPaseが存在し, しかもCa2+調節ホルモンである活性型ビタミンD3の1,25(OH)2D3によってHPLFのALPase活性が上昇すると報告している.
Practice　： 歯科学
Keywords　： HPLF, 細胞接着・伸展因子, FN, VN

English

Title　： Specificity of Cell Attachment and Spreading Factors of Human Periodontal Ligament Fibroblasts
Subtitle　：
Authors　： Shozo AMINO, Toshio KAWASE, Shigeru Saito
Authors（kana）　：
Organization　： Department of Oral Biochemistry, Kanagawa Dental College
Journal　： Kanagawa Shigaku
Volume　： 27
Number　： 2
Page　： 179-192
Year/Month　： 1992 / 9
Article　： Original article
Publisher　： Kanagawa Odontological Society
Abstract　： Abstract: Interactions between the extracellular cell attachment-spreading factors and periodontal ligament fibroblasts are thought to modulate the remodeling and regeneration of periodontium. Previous studies have investigated that human periodontal ligament fibroblasts (HPLF) at confluent phase synthesized the cell attachment and spreading factors (CASFs) in the conditioned medium (HPLF-CM). The purpose of this study is to characterize the CASFs by the reactivity to the antibodies of cell attachment factors and their receptors. HPLF obtained by the explant method described by T. Kawase and S. Saito were maintained with D-MEM supplemented with 5% FCS, 50μg/ml ascorbic acid and antibiotics. After a confluent phase of cultured HPLF, the medium was replaced with FCS-free MCDB 107 medium. HPLF-CM was harvested and centrifuged to remove cellular debris. The HPLF-CM and their fractions by PO-60K column were coated on the hydrophobic wells, then HPLF were cultured for 12hrs. After treatment by the antibodies of fibronectin (FN), vitronectin (VN), FN receptor (FNR) and VN receptor (VNR) for 12hrs, cells were morphologically observed with a phase contrast microscope. The HPLF-CM and 50kDa CASFs were applied to a hydroxyapatite (HA) column. The CASF activity of HA binding fractions was investigated and then the electrophoretic protein patterns of each fraction were observed by the method of SDS-PAGE. After HPLF-CM was separated by the gel chromatography of Sephacryl S-300 and ion exchange chromatography oy IEC-DEAE, CASFs assay of each eluted fraction was carried out. The CASF activity of HPLF-CM was not inhibited by the treatment of antibodies against FN, VN, FNR and VNR. The HA affinity molecules of HPLF-CM and 50kDa CASFs showed the CASF activity. SDS-PAGE revealed that HA chromatography fraction of 50kDa CASFs eluted by 0.5M phosphate buffer contained a protein with molecular weight 70kDa as a major component. Furthermore, it was observed that HPLF-CM consisted of the various CASFs which were separated by Sephacryl S-300 and IEC-DEAE chromatographies. Therefore, HPLF-CM could contain the various cell attachment and spreading factors which were not identical with FN and VN which are typical cell attachment factors in the extracellular matrix. We conclude that CASFs in HPLF-CM may play a important role in the formation and regeneration of periodontium by HPLF attachment and differention to root surfacees.
Practice　： Dentistry
Keywords　： HPLF, FN, VN