アブストラクト（27巻3号：神奈川歯学）

神奈川歯学

Japanese

Title　： LPS刺激に伴うヒト歯根膜線維芽細胞の炎症性サイトカイン遺伝子の発現
Subtitle　： 原著
Authors　： 山地矢須子, 梅本俊夫
Authors（kana）　：
Organization　： 神奈川歯科大学口腔細菌学教室
Journal　： 神奈川歯学
Volume　： 27
Number　： 3
Page　： 350-363
Year/Month　： 1992 / 12
Article　： 原著
Publisher　： 神奈川歯科大学学会
Abstract　： 「緒言」歯周疾患は炎症局所における組織破壊と歯槽骨の吸収に特徴付けられる疾患であり, 歯周病原細菌の感染に起因すると考えられている. 成人性歯周炎の病因菌としては, 患者の歯周ポケットから高頻度に分離されることから黒色色素産生性グラム陰性桿菌が注目されており, とくに, 歯周炎患者の血清中の抗体価が高いことなどからPorphyromonas gingivalis(P.gingivalis)が有力視されている. P.gingivalisの有する病原因子については多くの報告がある. 本菌はタンパク分解酵素を産生しており, 特にコラゲナーゼは歯周組織を直接破壊する因子として, またトリプシン様酵素はキニンカスケードを活性化することにより, 毛細血管の透過性を亢進し, 炎症を惹起する因子として注目されているさらに内毒素(Lipopolysaccharide : LPS)は, 宿主の免疫系細胞を刺激して間接的に組織を傷害する有力な病原因子と考えられている.
Practice　： 歯科学
Keywords　： HPLF, LPS, Cytokine Gene expression

English

Title　： Inflammatory Cytokine Gene Expression in Human Periodontal Ligament Fibroblast Stimulated with Bacterial Lipopolysaccharides
Subtitle　：
Authors　： Yasuko YAMAJI, Toshio Umemoto
Authors（kana）　：
Organization　： Department of Oral Microbiology Kanagawa Dental College
Journal　： Kanagawa Shigaku
Volume　： 27
Number　： 3
Page　： 350-363
Year/Month　： 1992 / 12
Article　： Original article
Publisher　： Kanagawa Odontological Society
Abstract　： Abstract ： Porphyromonas gingivalis (P.gingivalis) has been believed to be an important periodontopathic bacteria, however the role of P.gingivalis in pathogenesis of periodontal disease is not yet completely understood. On the other hand, it is well known that lipopolysaccharide (LPS) stimulate the production of various cytokines from leukocytes and tissue cells. Therefore, the aim of this study was to examine the effects of P.gingivalis LPS (P-LPS) on the induction of inflammatory cytokines mRNA expression in human periodontal ligament fibroblast(HPLF). P-LPS was prepared from P.gingivalis strain 381 by hot-phenol water extraction methods. HPLF cells were obtained from explant culture of periodontal ligament of healthy human extracted tooth. HPLF cells were sowed in culture plate at a concentration of 2～3×10 4 cells/cm2. Upon reaching confluence, HPLF cells were incubated with various dose of LPS from P.gingivalis or E.coli for 24h. After incubation, total cellular RNA was extracted by LiC1 procedure. Gene expression of various cytokines were analized by RT-PCR method or northern blotting analysis. The IL-6 and IL-8 synthesis were measured by ELISA assay. P-LPS suppressed alkaline phosphatase activity of HPLF cells, however showed no influence on cell number. The mRNA of IL-6, IL-8 and TGF-βwere expressed in HPLF cells, but IL-1α and β, TNF-α, TGF-α and GM-CSF were not detected by RT-PCR method. The expression of TGF-β mRNA was not influenced by treatment of both LPS. By the stimulation of P-LPS (＞1μg/ml) and 100μg/ml of E-LPS, IL-6 and IL-8 mRNA were greatly expressed compared with control by northern blotting analysis. The synthesis of IL-6 and IL-8 were also stimulated by P-LPS at various concentration but not by E-LPS. Secretion of IL-6 and IL-8 into the culture media started after 6h and 3h expose to 10μg/ml P-LPS, respectively. These results suggest that P.gingivlais may lead tissue destruction and bone resorption through IL-6 and IL-8 released from HPLF cells stimulated with its LPS.
Practice　： Dentistry
Keywords　：