アブストラクト(27巻4号:神奈川歯学)

神奈川歯学

Japanese

Title : コラーゲン・ゲル内培養におけるヒト歯根膜由来線維芽細胞の細胞接着・伸展因子の影響について
Subtitle : 原著
Authors : 川村太助, 川瀬俊夫, 鈴木善久, 宮間幹夫, 新井信明, 石井信義, 吉野練太朗, 斎藤滋
Authors(kana) :
Organization : 神奈川歯科大学口腔生化学教室
Journal : 神奈川歯学
Volume : 27
Number : 4
Page : 543-558
Year/Month : 1993 / 3
Article : 原著
Publisher : 神奈川歯科大学学会
Abstract : 「緒言」歯根膜は, 間歇的咬合力を受ける結合組織である. また, セメント質と歯槽骨という石灰化組織に囲まれ, 発生学的にセメント質, 歯槽骨と同様, 歯小嚢由来であるにも関わらず石灰化能が抑制された高度に分化した組織と考えられる. また, 機械的外力に対応して, 歯周疾患治癒における再生や再付着においても重要である. 近年, Saito, S.et al., Kawase, T.et al.と川瀬らにより, 歯根膜由来の線維芽細胞(Human Periodontal Ligament Fibroblast : HPLF)の培養系が確立され, この細胞の特徴からosteoblastic fibroblastと提唱された. 更に, Kawase, T.et al.は, HPLFにbone/liver/kidney-type ALPaseが存在し, しかもCa2+調節ホルモンである活性型ビタミンD3(1,25(OH)2D3)によってALPase活性が上昇し, 青木は, 1,25(OH)2D3によるHPLFのalkaline phosphatase(ALPase)上昇はKm値が一定で, Vmaxの上昇から酵素分子数の増加によるもので, SDS-PAGEのALPase活性染色から1,25(OH)2D3依存ALPaseの存在を示唆している.
Practice : 歯科学
Keywords : ヒト歯根膜由来線維芽細胞(HPLF)コラーゲン・ゲル内培養, ゲル収縮細胞接着・伸展因子

English

Title : Effect of Cell Attachment and Spreading Factors for Human Periodontal Ligament Fibroblasts within Collagen Gel Culture
Subtitle :
Authors : Taisuke KAWAMURA, Toshio KAWASE, Yoshihisa SUZUKI, Mikio MIYAMA, Nobuaki ARAI, Nobuyoshi ISHII, Rentarou YOSHINO, Shigeru SAITO
Authors(kana) :
Organization : Department of Oral Biochemistry, Kanagawa Dental College
Journal : Kanagawa Shigaku
Volume : 27
Number : 4
Page : 543-558
Year/Month : 1993 / 3
Article : Original article
Publisher : Kanagawa Odontological Society
Abstract : Abstract : We have reported that the osteoblastic characteristics of human periodontal ligament fibroblast(HPLF)were observed by two-dimensional culture system, which is established as a monolayer culture by many researchers. However, the fibroblasts of conective tissues are localized in the three-dimensional environment in vivo. Therefore, the purpose of this study was to evaluate the biological regulation of HPLF cultured in the collagen gel. These HPLF were inoculated at a cell density of 5.0×10 4 cells/cm3 with a reconstruction buffer, a 3mg/ml collagen solution, and a two-fold concentration of D-MEM containing 2mg GFS(growth factor in serum)/ml, 50μg ascorbic acid/ml, penicillin and streptomycin, in 24-petri multi wells. Cell shape in collagen gels showed long and narrow bipolar forms. The shape did not change when the conditioned medium HPLF(HPLF-CM)was added to the collagen gel contraction by addition of HPLF-CM showed significant difference compared with one by no addition of HPLF-CM. This contraction caused detachment of the gel from the wall. There was a specific fraction that produced gel contraction in HPLF-CM. The fraction that did not have affinity with the gelatin actually enhanced gel contraction. The fraction that did not have affinity with fetal bovine serum(FBS)in HPLF-CM also enhanced gel contraction. These results suggest that gel contraction was enhanced by cell attachment and spreading factor, while it was induced by cell-matrix interactions
Practice : Dentistry
Keywords :