アブストラクト(28巻2号:神奈川歯学)

神奈川歯学

Japanese

Title : Porphyromonas gingivalisの血球凝集活性に関与する遺伝子とその産生物の同定
Subtitle : 原著
Authors : 高橋祐介, 梅本俊夫
Authors(kana) :
Organization : 神奈川歯科大学口腔細菌学教室
Journal : 神奈川歯学
Volume : 28
Number : 2
Page : 117-128
Year/Month : 1993 / 9
Article : 原著
Publisher : 神奈川歯科大学学会
Abstract : 「緒言」Porphyromonas gingivalisは血液寒天培地上において黒色の集落を形成する口腔常在のグラム陰性, 無芽胞, 嫌気性桿菌で, 進行性歯周炎の病巣から高率かつ高頻度に分離されることおよび本菌の増加と歯槽骨破壊の急激な進行との間に相関が見られることなどから成人性歯周炎に関与するもっとも有力な細菌のひとつと考えられている. 本菌の歯周炎の発症や進行に関与する病原因子としてコラゲナーゼやトリプシン様酵素などのプロテアーゼ, ヘパリナーゼ, ヒアルロニターゼ, リボヌクレアーゼおよびデオキシリボヌクレアーゼなど様々な分解酵素が指摘されており, なかでもコラゲナーゼは直接的に歯根膜や歯肉の結合組織を破壊する可能性が示唆されている. またインドール, アンモニア, 硫化水素, メチルメルカプタン, および酪酸等の低分子代謝産物も組織障害性を示すことが報告されている.
Practice : 歯科学
Keywords : Porphyromonas gingivalis, 血球凝集, 組換えDNA

English

Title : Identification of Gene and Its Product Associate with Hemagglutinating Activity of Porphyromonas gingivalis
Subtitle :
Authors : Yusuke TAKAHASHI, Toshio Umemoto
Authors(kana) :
Organization : Department of Oral Microbiology, Kanagawa Dental College
Journal : Kanagawa Shigaku
Volume : 28
Number : 2
Page : 117-128
Year/Month : 1993 / 9
Article : Original article
Publisher : Kanagawa Odontological Society
Abstract : Abstract: There have been many reports concerned with the hemagglutination factor (s) of Porphyromonas gingivalis. However, the relation between hemagglutinating activity and the virulence of this organism has not been clear. In this study, one of the hemagglutinin genes that is located on a 3.3-kb BamHI-SalI fragment of a recombinant plasmid pNH37, which was constructed by insertion of a DNA fragment of P. gingivalis KD-1 into plasmid vector pBluescript II KS+ (N. Hamada. 1992 Kanagawa Shigaku; 26: 341-352.), was further analyzed. The BamHI-SalI fragment and several deletion fragments as well were then subcloned into the same vector. Escherichia coli K38/pGP1-2 was transformed with these plasmids and analyzed by T7 RNA polymerase/promotor system for hemagglutinating activity (HA) and for protein production. All HA-positive strains produced a 55-kDa protein that reacts to an antiserum against the culture supernatant of P. gingivalis KD-1. By sequencing of the fragment, presence of a 1547bp-long open reading frame (ORF) encoding a 56.3-kDa protein, was demonstrated. This protein may corresporld to the "55K gene product" suggested previously. The codon usage of this ORF was different from that of the genoms of E. coli. The codons rarely used in E. coli such as TTG-Leu, CTT-Leu, CCT-Pro, AAT-Asn, AAG-Lys and ACG-Thr were found to be frequently used in this organism. Although typical signal peptide was not found in the N-terminal of this protein, its secretion to the outer membrane of E. coli could be detected. Dot blot analysis revealed that P. gingivalis strains KD-1, FDC381, ATCC33277, SU63, SU60, W83, W50 contained this gene, however no DNA homology between P. gingivalis and other black-pigmented species such as P. asaccharolitica ATCC25260, ATCC27067, P. endodontalis ATCC35406, P. intermedia ATCC25261, P. melaninogenica ATCC25845, B. levii ATCC29147 species could be detected.
Practice : Dentistry
Keywords : Porphyromonas gingivalis