アブストラクト(28巻2号:神奈川歯学)

神奈川歯学

Japanese

Title : 口腔前癌病変の悪性化潜在能に関する研究 - 核DNA量, 核面積の細胞測光による検討 -
Subtitle : 原著
Authors : 井上聡, 志村介三
Authors(kana) :
Organization : 神奈川歯科大学口腔外科学教室第II講座
Journal : 神奈川歯学
Volume : 28
Number : 2
Page : 185-199
Year/Month : 1993 / 9
Article : 原著
Publisher : 神奈川歯科大学学会
Abstract : 「緒言」前癌病変の悪性潜在能を的確に把握することは, 疾患の予後を予測し, 適切な治療法を行うために重要である. この点に関して, これまで, 主に臨床, 病理学的検討がなされ, とくに上皮異形成と癌化との関連性が指摘されている. しかし, 組織学的な評価には検索者の主観が入りやすいこと, また, 実際に上皮異形成のみられないものでも, 癌化をきたすこともあり, 悪性潜在能に対するより生物学的な客観的指標が求められている. 近年, 細胞測光法の進歩により種々の臓器腫瘍において, 核DNAの定量解析が行われるようになり, 核DNA量の分布パターンが, 良性, 悪性の鑑別や腫瘍の生物学的性状の指標として注目されている. しかし口腔の前癌病変に関する系統的な研究は少なく, また核DNA量と核の大きさのパラメーターによる分析はほとんどみられない.
Practice : 歯科学
Keywords : 口腔前癌病変, 白板症, 紅板症, 核DNA量, 核面積

English

Title : Study on the Potentiality of Malignant Transformation in Oral Precancerous Lesion - Nuclear DNA Content and Nuclear Area -
Subtitle :
Authors : Satoshi INOUE, Kaizo Shimura
Authors(kana) :
Organization : The Second Department of Oral and Maxillofacial Surgery, Kanagawa Dental College
Journal : Kanagawa Shigaku
Volume : 28
Number : 2
Page : 185-199
Year/Month : 1993 / 9
Article : Original article
Publisher : Kanagawa Odontological Society
Abstract : Abstract: This study was made in order to obtain objective indices for predicting the transformation of oral precancerous lesions into cancer and distinguishing them from eary stage cancer. For this purpouse, the nuclear DNA content in the cells of oral precancerous lesions and their nuclear area were quantified, and examined in comparison with those for squamous epithelia of 18 normal oral mucosa and 80 squamous cell carcinomas. Samples of oral precancerous lesion were taken from 26 cases of leukoplakia and 4 cases of erythroplakia. The findings are as follows: (1) The nuclear DNA histogram of normal oral mucosa showed a diploid pattern with a modal peak in the 2C region (1.5C-2.4C). The frequency of cells above 4C was 3% or less. The mean DNA content was 1.88C (+-0.20), its variance being 0.18 (+-0.14). The mean nuclear area stood at 41.1μ2 (+-7.66), its variance 57.6 (+-20.3). The scattergram of nuclear DNA content and nuclear area showed that the cells accumulate Within the range from 30μ2 to 50μ2. (2) The DNA histogram of oral squamous cell carcinoma showed a diploidy pattern in 31 cases (39%). However, 49 cases (61%) were an aneuploidy pattern that have modal peak outside the 2C region or more than one peak. The nuclear DNA content and nuclear area were deviant from the normal range. The mean DNA content came at 3.4C (+-0.70) and variance 1.71C (+-1.16). The frequencies of hyperploid cells above 4C ranged from a minimum 4% and a maximum 80%, averaging 26% (+-19.5). The mean nuclear area was 86.8μ2 (25.3%), its variance being 929 (+-813). (3) Out of the 26 leukoplakia cases, 23 cases (88%) showed a diploidy pattern on the DNA histogram and 3 cases (12%) an aneuploidy pattern. The scattergrams of the nuclear DNA content and nuclear area ahowed various patterns; some were similar to that of normal oral mucosa and some like that of squamous cell carcinoma, showed a atendency to disperse. The mean nuclear DNA content was 2.34C (+-0.39), its variance being 0.42 (+-0.34). The frequency of hyperploid cells above 4C was 5.02% (+-5.92). The mean nuclear area was 50.2μ2 (+-16.7), its variance being 189 (+-228). (4) the nuclear DNA histogram of erythroplakia showed a diploidy pattern in two cases (50%) and an aneuploidy pattern in the remaining 2 cases. In the scattergram, 3 cases showed a notable dispersion analogous to the scattergram of squamous cell carcinoma. The mean DNA content was 2.82C (+-0.28). Its variance being 1.42 (+-0.79). The frequency of cells above 4C was 13.6% (+-8.06). The mean nuclear area was 79.6μ2 (+-15.9%), its variance being 981 (+-532). (5) In discriminat analysis of normal mucosa and squamous cell carcinoma with these values mentioned above being variables, the two variances for the mean DNA content and the mean nuclear area were proved to be siganificant. (6) From the discriminant function obtained in the present study, It was determined that 15 cases out of total 26 leukoplakia cases should belong the normal group, and the remaining 11 cases and all the erythroplakia cases be included in the cancer group. Cases of the normal group had not transformed into malignancy since resection, but of the malignat group, one resected case, one case vaporized by Laser, one radiation therapy case and case under observation have later developed into carcinoma. In conclusion, the multivariate analysis of the nuclear DNA content and nuclear area of oral precancerous lesions has yielded impotant results that should be considered to be of clinical value as criteria and diagnosis of eary cancer.
Practice : Dentistry
Keywords :