アブストラクト（28巻3号：神奈川歯学）

神奈川歯学

Japanese

Title　： o-Phthalaldehydeを用いた根管内清掃度検査法 [II] (簡易蛍光分光光度計の応用)
Subtitle　： 原著
Authors　： 白井夏野, 長田保
Authors（kana）　：
Organization　： 神奈川歯科大学歯科保存学教室第III講座
Journal　： 神奈川歯学
Volume　： 28
Number　： 3
Page　： 322-335
Year/Month　： 1993 / 12
Article　： 原著
Publisher　： 神奈川歯科大学学会
Abstract　： 「緒言」古くから根尖病巣成立の最も大きな原因は, 根管内に生息する細菌であると言われてきた. そのため根管治療終了の客観的目安として根管内細菌培養試験が広く行われてきた. しかし, 近年の免疫学的分析法の発展に伴って, 根尖性歯周疾患の起因物質は細菌ばかりではなく根管内に存在する抗原物質によっても惹起され進展していくと示唆する多数の報告がなされている. この抗原物質とは主に抜髄後の根管内に残存した歯髄や血液成分, また, 感染根管内の壊死歯髄片, 根尖部からの出血, 濃汁および滲出液などが貯留変性したものであり, 根尖部歯周組織での免疫応答の結果, 炎症を引き起こすと考えられている. このように多くの根尖病変成立に関する基礎的研究がなされ, 起因物質が判明してきたにもかかわらず, 臨床の場においてはこれらの研究成果を考慮した治療法がなされていないのが現状である.
Practice　： 歯科学
Keywords　： o-Phthalaldehyde, 簡易蛍光分光光度計, 根管内清掃度

English

Title　： The Evaluation of Root Canal Cleanliness Test with o-Phthalaldehyde [II]
Subtitle　：
Authors　： Natsuno SHIRAI, Tamotsu Osada
Authors（kana）　：
Organization　： Department of Endodontics, Kanagawa Dental College
Journal　： Kanagawa Shigaku
Volume　： 28
Number　： 3
Page　： 322-335
Year/Month　： 1993 / 12
Article　： Original article
Publisher　： Kanagawa Odontological Society
Abstract　： Abstract: Recently, numerous studies have suggested that the necrotic pulp and breakdown products of degenerating tissue appear to be a reservoir of antigens to periapical tissue such as bacteria. KODAMA has developed a new root canal cleanliness test to detect contamination where there are no bacteria in the root canal. This test uses o-Phthalaldehyde (OPA) reagent which produces a sensitive fluorescence reaction to protein and amino acid. The fluorescence intensity has been determined visually. The purpose of this study is to develop a spectrofluorometer to measure fluorescence in the test. The device is evaluated experimentally and clinically. The results are summarized as follows. 1. The temperature in the sample chamber of the device Is held constantly at 30～31℃. 2. The fluorescence intensity of OPA which was reacted with a protein (human albumin) of various concentrations was continuously measured for one hour. It is shown that the fluorescence reduced with time. However, if the fluorescence is measured at an exact known elapsed time, it is possible to determine the quantity of protein at each concentration. 3. After endodontic treatment, using human extracted teeth, the cleanliness of the root canal was evaluated by stereomicroscopy, SEM, and fluorescence intensity measured with the experimental device. 1) The stereomicroscopy and SEM findings showed that the debris in the root canal was diminished by learger instrumentatien. Fluorescence intensity was reduced when the remaining equal debris was decreased. 2) The sampling method was to fill the root canal with distilled water and slightly scrape the canal surface with a #20 H-file. Then, a sterile absorbent point was inserted into the canal for at least a minute and then dropped into the OPA reagent. 3) A reading of less than 100 on the device corresponded to a canal nearly free of contamination. 4. The clinical study mainly examined the relation between the OPA test and a microbial culture test. The result indicated that this test is more useful in detecting contamination of the canal than the microbial culture test commonly used in endodontics.
Practice　： Dentistry
Keywords　： o-Phthalaldehyde