アブストラクト(28巻4号:神奈川歯学)

神奈川歯学

Japanese

Title : ヒト乳歯および永久歯歯根膜由来線維芽細胞に関する細胞生化学的研究 - 細胞増殖と分化に対するretinoic acidおよび機械的外力の影響について -
Subtitle : 原著
Authors : 長谷川知史1),2), 大西武1),2), 木本茂成1),2), 檜垣旺夫1), 斎藤滋2)
Authors(kana) :
Organization : 1)神奈川歯科大学小児歯科学教室, 2)神奈川歯科大学口腔生化学教室
Journal : 神奈川歯学
Volume : 28
Number : 4
Page : 440-451
Year/Month : 1994 / 3
Article : 原著
Publisher : 神奈川歯科大学学会
Abstract : 「緒言」 健全な咬合の育成ならびに口腔機能の獲得を目的とする小児歯科学領域において, 成長発育の過程での歯および歯周組織の形成と改造の現象を細胞生物学的に解明することは必要不可欠である. 歯周組織の一部を構成する歯根膜は咬合や咀嚼による機械的外力を歯から顎骨へと伝達する重要な組織であり, 歯根の形成や歯の萌出に関与していると考えられる. 特に乳歯歯根膜は歯根の形成と吸収, 歯の萌出に伴う乳歯歯周組織の変化, さらに成長発育の過程における歯槽骨の形態変化の際にも, 直接あるいは関接的に重要な役割を果たしている. したがって, 乳歯歯根膜組織が関与すると考えられる現象に影響を与える困子を同定するためには, 乳歯および永久歯歯根膜組織由来の培養細胞を用いて両者の細胞生物学的相違を検索することが必要である. Saito, Kawaseらは歯根膜組織のexplantによりヒト永久歯歯根膜線維芽細胞(HPLF)の培養系を樹立し, その細胞生物学的性質の検索を行い, HPLFはvitamin D dependentに発現するalkaline phosphatase (ALPase)を産生することを報告した.
Practice : 歯科学
Keywords : 歯根膜線維芽細胞, retinoic acid, 機械的外力

English

Title : Cytobiochemical Study on Human Periodontal Ligament Fibroblasts Derived from Deciduous and Permanent Teeth - Effects of Retinoic Acid and Mechanical Stress on Cell Growth and Differentiation -
Subtitle :
Authors : Satoshi HASEGAWA1),2), Takeshi ONISHI1),2), Shigenari KIMOTO1),2), Morio Higaki1), Shigeru Saito2)
Authors(kana) :
Organization : 1)Department of Pediatric Dentistry, Kanagawa Dental College, 2)Department of Oral Biochemistry, Kanagawa Dental College
Journal : Kanagawa Shigaku
Volume : 28
Number : 4
Page : 440-451
Year/Month : 1994 / 3
Article : Original article
Publisher : Kanagawa Odontological Society
Abstract : Abstract : The effects of retinoic acid (RA) and mechanical stress on cell growth and differentiation were studied on periodontal ligament fibroblasts derived from human deciduous (HPLF-Y) and permanent (HPLF) teeth in culture. HPLF-Y and HPLF were treated with RA at the range of 10-7 to 10-5 M for 8 days, and also treated with RA or with RA and 5 nM 1,25 dihydroxy vitamin D3 (D3) together. Both cells were loaded with mechanical strain intermittently using the flexible bottomed culture plates and vacuum system. DNA synthesis of HPLF-Y and HPLF were suppressed by treatment with 0.1μM RA for 8 days. The growth of both cells were more suppressed by RA treatment in the latter half of culture period than by D3 or by RA and D3 together. Alkaline phosphatase (ALPase) activity of HPLF-Y was increased by treatment with 0.1μM RA for 8 days, however no significant change was observed on HPLF after treatment with RA at the same concentration. After the loading of intermittent stretching (5%, 3 cycle/min, 24hr) on the day 5 in culture, cell growth of HPLF was stimulated, whereas that of HPLF-Y was suppressed. On the other hand, ALPase synthesis was stimulated on both HPLF-Y and HPLF. The mechanical stress (5%) caused significant increase of ALPase on HPLF-Y treated with D3 or with RA and D3 together at confluent phase. ALPase activity was increased by stretching at growth phase of HPLF treated with RA or D3 separately or with RA and D3 together. These data demonstrate that there are obvious differences between HPLF-Y and HPLF in terms of the response so RA or mechanical stress. It is that HPLF-Y and HPLF have different functions in the remodeling of periodontal tissue during development.
Practice : Dentistry
Keywords : retinoic acid