アブストラクト(29巻3号:神奈川歯学)

神奈川歯学

Japanese

Title : Selenomonas sputigenaから分離した内毒素リポ多糖の性状
Subtitle : 原著
Authors : 外村誠, 熊田秀文, 渡辺清子, 梅本俊夫
Authors(kana) :
Organization : 神奈川歯科大学口腔細菌学教室
Journal : 神奈川歯学
Volume : 29
Number : 3
Page : 189-198
Year/Month : 1994 / 12
Article : 原著
Publisher : 神奈川歯科大学学会
Abstract : 「緒言」 近年, ある種の特異的な細菌あるいは細菌叢により異なった病型の歯周炎が惹起されることが示唆されている. 進行性歯周炎の原因菌としてはPorphyromonas gingivalis, 急性壊死性潰瘍性歯肉炎の原因菌としてはPrevotella intermediaやSpirochetes, また若年性歯周炎の病原菌としてはActinobacillus actinomycetemcomitansが考えられている. 一方, 活動期の進行性歯周炎では歯肉溝内にSpirochetes, Campylobacter (Wolinellaを含む)およびSelenomonasなどの運動性桿菌の割合が著しく増加することが多くの研究者により報告されている. Listgarten & Helldenは運動性桿菌と非運動性桿菌の割合を暗視野顕微鏡を用いて検討し, 健康部位では1:49であるが, 病巣部位では約1:1と運動性桿菌の比率が明らかに増加することを報告している.
Practice : 歯科学
Keywords : Selenomonas sputigena, 内毒素リポ多糖, (LPS), 化学性状, 生物活性

English

Title : Characterization of Lipopolysaccharide Isolated from Selenomonas sputigena
Subtitle :
Authors : Makoto TONOMURA, Hidefumi KUMADA, Kiyoko WATANABE, Toshio Umemoto
Authors(kana) :
Organization : Department of Oral Microbiology, Kanagawa Dental College
Journal : Kanagawa Shigaku
Volume : 29
Number : 3
Page : 189-198
Year/Month : 1994 / 12
Article : Original article
Publisher : Kanagawa Odontological Society
Abstract : Lipopolysaccharide (LPS) was isolated and purified from Selenomonas sputigena ATCC 33150 by the phenol/water procedure, and treatments of DNase, RNase, α-amylase and pronase. The sugar components of the LPS were mannose, galactose, glucose, L-glycero-D-manno-heptose, 2-keto-3-deoxyoctonate (KDO), glucosamine and galactosamine. The degraded polysaccharide prepared from the LPS by mild acid hydrolysis was fractionated by Sephadex G-50 gel chromatography into three fractions : (1) a middle-molecular-mass fraction consisting of a short side chain of galactose and glucosamine (6 and 2 mols per 3 mols of heptose residue) with attached core oligosaccharide ; (2) a core oligosaccharide containing heptose, glucose, glucosamine and phosphate ; (3) a low-molecular-mass fraction containing galactosamine, KDO and phosphate. The main fatty acids of lipid A were hendecanoic acid, tridecanoic acid, tridecenoic acid, 3-hydroxytridecenoic acid and 3-hydroxytetradecanoic acid. The biological activities of the LPS were similar to those of Salmonella typhimurium LPS in activation of the clotting enzyme of Limulus amoebocytes, the Schwartzman reaction, mitogenicity for murine lymphocytes and production of IL-lα and IL-6 for murine macrophages, although some of the biological activities of lipid A were lower than those of intact LPS.
Practice : Dentistry
Keywords : Selenomonas sputigena