アブストラクト（29巻3号：神奈川歯学）

神奈川歯学

Japanese

Title　： ラット顎下腺の分化過程におけるprogrammed cell deathの役割
Subtitle　： 原著
Authors　： 加藤浩一1),2), 佐々昭三1), 高橋和人2)
Authors（kana）　：
Organization　： 1)神奈川歯科大学口腔組織学教室, 2)神奈川歯科大学口腔解剖学教室
Journal　： 神奈川歯学
Volume　： 29
Number　： 3
Page　： 199-216
Year/Month　： 1994 / 12
Article　： 原著
Publisher　： 神奈川歯科大学学会
Abstract　： 「緒言」 出生時のラット顎下腺は, 未発達な導管系とその終末部であるterminal tubuleから構成されており, 細胞の分化・増殖の過程を経て, 成熟腺組織構造へと発達することが知られている. 成熟腺組織の腺房に相当するterminal tubuleは幼若期のsecretory unitであり, terminal tubule cell (TT-cell)およびproacinar cell (PA-cell), acinar cell (A-cell)の3種の分泌細胞によって構成されている. このうちTT-cellとPA-cellは顎下腺の発達に伴って消退することから, 顎下腺の分化発達過程に関する多くの研究がなされている. PA-cellはA-cellのprecursor cellとして生後発達の早期に消退することが確かめられており, また, TT-cellは出生時のterminal tubuleの主体を占めており, 離乳期ではこの分泌細胞のみによって構成された腺管が, 介在部導管と終末部との間に存在する.
Practice　： 歯科学
Keywords　： submandibular gland, programmed cell death, apoptosis, Le y antigen

English

Title　： Study on Role of the Programmed Cell Death in the Developing Submandibular Gland of Rat
Subtitle　：
Authors　： Hirokazu KATO1),2), Shozo Sasa1), Kazuto Takahashi2)
Authors（kana）　：
Organization　： 1)Department of Oral Histology, Kanagawa Dental College, 2)Department of Oral Anatomy, Kanagawa Dental College
Journal　： Kanagawa Shigaku
Volume　： 29
Number　： 3
Page　： 199-216
Year/Month　： 1994 / 12
Article　： Original article
Publisher　： Kanagawa Odontological Society
Abstract　： Recent studies have suggested the close association of apoptosis with the differentiation and development of the organ. This report evaluated the postnatal growth of the rat submandibular gland by light microscopy, electron microscopy, an immunohistochemical method using anti-Lewis Y antibody (Ley), which is an apoptosis associated antibody, and the nick end labeling method. The distal portion of the prepubertal submandibular gland consists of terminal tubules, an immature secretory unit. In this area, 3 types of cells were observed: acinar cells, proacinar cells (PA-cells), and terminal tubule cells (TT-cells). There were an intercalated duct and a striated duct continuously with the terminal tubules. Electron microscopy showed the absence of PA-cells until 14 days after birth. Le y revealed positive reactions only on the luminal surface of the cells in the intercalated duct until 14 days after birth. The nick end labeling method showed negative reactions in all cells until 14 days after birth. Light microscopy showed pyknosis, suggesting apoptosis 18-21 days after birth. Le y showed a strongly positive reaction in many intercalated duct cells and some TT-cells 18 days after birth. This reaction was observed in the entire cytoplasm excluding the nucleus. The number of strongly positive cells gradually decreased until 33 days after birth, and positive reactions were observed only in the luminal surface membrane of cells in the intercalated duct. The nick end labeling method showed positive reactions in a small number of cells in the intercalated duct and TT-cells. The number of positive cells increased thereafter, reaching a peak 28 days after birth. Electron microscopy revealed nuclear chromatin aggregation, suggesting apoptosis in intercalated duct cells and TT-cells 21 days or more after birth and phagocytosis of the apoptotic body by these cells. These results suggest that the close involvement of apoptosis in the regulation of cells constituting the submandibular gland in the postnatal growth of the rat submandibular gland. The inconsistency among findings obtained by Le y, the nick end labeling method, and electron microscopy may reflect different stage of the development of apoptosis.
Practice　： Dentistry
Keywords　： submandibular gland, programmed cell death, apoptosis, Le y antigen