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アブストラクト（29巻3号：神奈川歯学）
Japanese
| Title ： | PCR法による歯からの性別判定 - とくに海水と土中の影響について - |
|---|---|
| Subtitle ： | 原著 |
| Authors ： | 宇都宮丈児, 山本勝一 |
| Authors（kana） ： | |
| Organization ： | 神奈川歯科大学法医学教室 |
| Journal ： | 神奈川歯学 |
| Volume ： | 29 |
| Number ： | 3 |
| Page ： | 217-226 |
| Year/Month ： | 1994 / 12 |
| Article ： | 原著 |
| Publisher ： | 神奈川歯科大学学会 |
| Abstract ： | 「緒言」 法医歯科学領域における身元不明死体の個人識別で最も重要なものは歯である. これは, 近年, 航空機事故などの大規模災害, また, 凶悪犯罪に関する白骨死体およびバラバラ死体など, 損傷の激しい遺体が発見されることが多く, これらの場合には, 硬組織である歯から個人識別を行うことが最も有効であり, その検屍方法は, 山本らによって報告されている. また, これらの遺体の場合には, 個人識別を行うにあたってなくてはならない要因のひとつである性別判定が不可能なことが多く見られる. そこで, 歯からの性別判定は, 従来から, 羽賀のエナメル質分光透過率および象牙質の比重, 権田, Ditch & Rose, Garn, et al.の歯の計測値, Dixon & Torr, 末永による歯髄細胞の性染色質(sex chromatin)の出現率, また, 石津ら, Senoによる歯髄細胞のY染色体の蛍光小体(F-body)からの出現率などの性別判定が報告されているが, 著者は, 歯髄からDNAを抽出し, Mullis & Faloona, Saiki, et al.によって発表されたpolymerase chain reaction(以下PCRと略す)を用いて性別判定をなった. |
| Practice ： | 歯科学 |
| Keywords ： | PCR法, 性別判定, Amelogenin locus, DYZ1 locus, DYZ3 locus |
English
| Title ： | Sex Determination by PCR Method of Teeth - Special Reference to Influence Sea-water and Underground - |
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| Subtitle ： | |
| Authors ： | Jouji UTSUNOMIYA, Katsuichi Yamamoto |
| Authors（kana） ： | |
| Organization ： | Department of Forensic Medicine, Kanagawa Dental College |
| Journal ： | Kanagawa Shigaku |
| Volume ： | 29 |
| Number ： | 3 |
| Page ： | 217-226 |
| Year/Month ： | 1994 / 12 |
| Article ： | Original article |
| Publisher ： | Kanagawa Odontological Society |
| Abstract ： | Dental pulp is evaluated to be the best source for extracting DNA molecules from severely damaged cadavers including drowned, burned and putrid cadavers, in the field of forensic dental medicine. On the basis of our experience that DNA fragments are difficult to extract from the dental pulps of cadavers, a fundamental investigation was conducted to elucidate the effect of various natural conditions such as the sea-water and the underground. Several teeth just extracted were immersed in sea-water or buried underground for 1, 2, 3, 4, 5, 6, 7, 14 and 28 days, after which the DNA was extracted from the dental pulps, and limits of successful sex determination from them were analyzed by using polymerase chain reaction (PCR) amplification method. In the experiment AMXY-1F and AMXY-2R, both corresponding to Amelogenin locus, were used. Sex determination was possible for all 7 teeth up to the 3rd day of immersion in the sea -water, and for 4,3,2 and 1 tooth on the 4th, 5th, 6th and 7th day of immersion, respectively. However, sex could not be determined on or after the 10th day. Sex determination was possible for all 7 teeth up to the 2nd day after burial underground, and 3 on the 3rd and 1 on the 4th day after burial. However, sex could not be determined on or after the 5th day. High molecular weight DNA could be extracted from the control teeth left at room temperature under a dry state for not only 28 days but also 2 years or more, as though the teeth had just been extracted, proving the efficiency of the PCR method for sex determination. Sex determination by the PCR method was more difficult using the teeth on the 4th day underground because the bulk of high molecular weight DNA had been degraded 2 days earlierthan in the sea-water. The possibility of sex determination is lowered because the molecular size of the DNA is reduced gradually in the sea-water, whereas underground it is reduced rapidly with the borderline on the 3rd day. There was no difference on the DNA detection rate depending on the subject's age nor there was difference between single and plural radicular pulps. The present study led us to conclude that : 1. Sex determination from the teeth immersed in the sea-water was possible until the 3rd day, but impossible on or after the 7th day because the molecular size of DNA was gradually reduced from the 4th day. 2. Sex determination from the teeth buried underground was possible until the 2nd day, but impossible on the 4th day because the molecular size of the DNA was rapidly reduced. 3. The DNA extraction from the teeth was more strongly affected by the underground condition than by the sea-water because the DNA was degraded 2 days earlier. 4. No difference was observed in the DNA extraction, between the kind and morphology of the teeth left either in the sea-water or underground. 5. Pure water affects the teeth earlier than physiological saline. 6. Sex determination by the PCR amplification method using ANXY-1F and AMXY-2R as primers, both corresponding to Amelogenin locus, is simple and highly reliable for manipulation. |
| Practice ： | Dentistry |
| Keywords ： | Amelogenin locus, DYZ1 locus, DYZ3 locus |
