アブストラクト（29巻3号：神奈川歯学）

神奈川歯学

Japanese

Title　： Porphyromonas gingivalisにおける線毛の多様性
Subtitle　： 原著
Authors　： 武田康篤, 浜田信城, 高橋祐介, 曽根秀明, 渡辺清子, 熊田秀文, 松島秀樹, 宮地良彰, 鈴木正之, 梅本俊夫
Authors（kana）　：
Organization　： 神奈川歯科大学口腔細菌学教室
Journal　： 神奈川歯学
Volume　： 29
Number　： 3
Page　： 261-269
Year/Month　： 1994 / 12
Article　： 原著
Publisher　： 神奈川歯科大学学会
Abstract　： 「緒言」 Porphyromonas gingivalis (P.gingivalis)は黒色色素産生性のグラム陰性嫌気性桿菌の一種であり, 歯周病患者のポケットから高率に分離されること, および本菌に対する血清抗体価が歯周病血清において有意に高い価を示すことから, 歯周病原細菌の有力候補の一つとして注目されている. 歯周炎の発症や進行には, P.gingivalisのコラゲナーゼやトリプシン様酵素など直接的に組織破壊を引き起こす可能性があるタンパク分解酵素や, 免疫細胞を活性化したり, 歯周組織を構成する細胞からサイトカインの産生を惹起する内毒素など, 様々な病原因子が関与すると考えられている. 菌体表層に繊維状構造物として存在する線毛も免疫細胞や歯周組織を刺激してサイトカインの産生を誘発する活性の他に組織細胞への付着性を介して本菌の歯周ポケット内への定着に重要な役割を果たしていると考えられている.
Practice　： 歯科学
Keywords　： Porphyromonas gingivalis, 線毛, 抗原性, 抗線毛モノクローナル抗体

English

Title　： Heterogeneity of Fimbriae in Porphyromonas gingivalis
Subtitle　：
Authors　： Yasuatsu TAKEDA, Nobushiro HAMADA, Yusuke TAKAHASHI, Hideaki SONE, Kiyoko WATANABE, Hidefumi KUMADA, Hideki MATSUSHIMA, Yoshiaki MIYAJI, Masayuki SUZUKI, Toshio UMEMOTO
Authors（kana）　：
Organization　： Department of Oral Microbiology, Kanagawa Dental College
Journal　： Kanagawa Shigaku
Volume　： 29
Number　： 3
Page　： 261-269
Year/Month　： 1994 / 12
Article　： Original article
Publisher　： Kanagawa Odontological Society
Abstract　： The fimbriae of Porphyromonas gingivalis are thought to be an important virulence factor in mediating adherence of the organism of the host tissues. It has been reported that there is heterogeneity in the molecular size, the N-terminal amino-acid sequence and antigenicity of the fimbrial proteins that were isolated from different strains of P.gingivalis. We have also noted that an antigenic divercity in fimbrial proteins of some strains is present using a monoclonal antibody (MAb) against the fimbriae of the strain 381. We also found that the strain SU63 did not react to the MAb against the fimbriae of the strain 381 by immunoblot analysis. In order to define this antigenic divercity of these fimbrial proteins, we purified it from the strain SU63 by DEAE Sepharose CL-6B column chromatography. The fimbriae were eluted with Tris-HCI buffer containing 0.15 M NaCl. The purified fimbriae produced two protein bonds of 41 kDa and 55kDa on SDS-PAGE. Subsequently, we prepered a murine MAb specific for this fimbrial protein. It specifically reacted only to the 41 kDa band of the fimbrial proteins of the same strain. No immunoreactive band, however, was detected from the strain ATCC 33277. Immunogold electron microscopy demonstrated that the anti-SU63 fimbriae MAb bound only to the short fimbria-like projections of the same strain. In contrast, no binding was observed with strain ATCC 33277. The amino acid sequence of the 21 residues on the amino N-terminal of the 41 kDa protein band from strain SU63 were determind by an amino acid sequencer 473A (PERKIN ELMER Co., Tokyo, Japan) which was then compared with the published data for seven strains of P.gingivalis. The sequence of the strain SU63 was distinctly different from those of the strains 381 or ATCC 33277. it was also shown to be almost identical to those of the strains 9-14k-1 or HG 564. Hence we concluded that the fimbriae of the strain SU63 belonged to a different antigenic group from those of the strains 381 or ATCC 33277.
Practice　： Dentistry
Keywords　： Porphyromonas gingivalis