アブストラクト（31巻3号：神奈川歯学）

神奈川歯学

Japanese

Title　： ヒト歯根膜由来線維芽細胞 (HPLF) の増殖と分化調節因子としてのアスコルビン酸の有効性に関する研究
Subtitle　： 原著
Authors　： 石井信義, 出口眞二*1, 川瀬俊夫*2, 斎藤滋
Authors（kana）　：
Organization　： 神奈川歯科大学口腔生化学教室, *1神奈川歯科大学歯周病学講座, *2神奈川歯科大学歯科生体工学教室
Journal　： 神奈川歯学
Volume　： 31
Number　： 3
Page　： 252-264
Year/Month　： 1996 / 12
Article　： 原著
Publisher　： 神奈川歯科大学学会
Abstract　： 「緒言」歯周疾患の有病率は, 歯冠修復の歯科医療の発展に反し依然として高いのが現状であり, 8020運動にもかかわらず, 有病者の悩みが十分解決されていない. 歯周疾患の病態は, Kornmanらが報告しているように多様である. 一方, 歯周疾患の治癒過程は, 細胞の遊走で始まり, 細胞外基質成分の合成で完結するが, 咀嚼機能が回復すると, 再生歯根膜は咬合力による機械的外力に応答した組織改造が始まる. KawaseらとSaitoらは歯周組織, 特にヒト歯根膜由来線維芽細胞(HPLF)の培養系を確立し, その細胞の特徴からHPLFを骨芽細胞様線維芽細胞(Osteoblastic fibroblasts)と呼称した. このHPLFに対し, 青木はビタミンD3によるALPaseの発現調節について検索し, 笹栗は骨に特徴的なタンパク質の発現を報告している. また, HPLFに対する機械的外力に関しては, Saitoらを始め, 川瀬ら, 岸, Kim, Yasuら, 川村, 川村ら, 吉野ら, がそれぞれ報告している.
Practice　： 歯科学
Keywords　： アスコルビン酸, HPLF, 増殖・分化

English

Title　： Effects of Ascorbic Acid as a Regulatory Factor of Proliferation and Differentiation of Human Periodontal Ligament Derived Fibroblasts (HPLF)
Subtitle　：
Authors　： Nobuyoshi ISHII, Shinji DEGUCHI*1, Toshio KAWASE*2, Shigeru Saito
Authors（kana）　：
Organization　： Department of Oral Biochemistry, Kanagawa Dental College, *1Department of Periodontology, Kanagawa Dental College, *2Department of Dental Bioengineering, Kanagawa Dental College
Journal　： Kanagawa Shigaku
Volume　： 31
Number　： 3
Page　： 252-264
Year/Month　： 1996 / 12
Article　： Original article
Publisher　： Kanagawa Odontological Society
Abstract　： [Abstract]: The aim of this study was to explore the effect of ascorbic acid (AsA) on the cellular responses of human periodontal ligament fibroblasts (HPLF) obtained by the explant method described by Kawase et al (Adv Dent Res 1988). We have demonstrated the effects of cell attachment and spreading factors (CASFs) on HPLF adhesion which play fundamental roles in the regeneration processes of periodontal ligament. In this experiment, L-ascorbic acid-2-phosphate (AsA-2P) which is stable in a solution was used. The cells were maintained with D-MEM supplemented with 5%FCS, 0.25mM AsA-2P and antibiotics. The HPLF cultured without AsA-2P for 7 days were used in the following experiments : 1) At a growth phase, HPLF were labeled with 0.37 MBq/ml 3H-thymidine (3H-TdR) in the culture medium containing 0.25mM AsA-2P and/or 2ng/ml TGF-β1 for 24 hrs. The cell growth was determined by the incorporation of 3H-TdR into cold TCA precipitates. 2) HPLF were exposed to AsA-2P and/or TGF-β1 until a confluent phase. The activity of alkaline phosphatase (ALPase) was determined using p-nitrophenylphosphate. 3) The confluent cells were incubated for 24 hrs in FCS-free media containing 1MBq/ml 35S-methionine (35S-Met) with AsA-2P and/or TGF-β1. Media proteins were separated through SDS-PAGE and contacted with X-ray film. 4) The conditioned medium of HPLF (HPLF-CM) treated with AsA-2P and/or TGF-β1 was assayed for the CASF activity and chemotactic activity. The increase of chemotactic activity in response to HPLF-CM of HPLF exposed to AsA-2P was significantly greater than that observed in response to TGF-β1, AsA-2P increased the cell growth and differentiation of HPLF to 1.56 fold and 3.78 fold, respectively. The fully differentiated HPLF cultured with AsA-2P and/or TGF-β1 synthesized and secreted the CASFs in a extracellular fluid. AsA-2P and TGF-β1 enhanced the synthesis of extracellular components of HPLF which were labeled with 35S-Met. Therefore, AsA-2P can modulate the regeneration processes of periodontal ligament categorized as 1) cell migration, 2) cell attachment and spreading, 3) cell proliferation and differentiation, and 4) the synthesis of extracellular matrix. AsA-2P may play a key role in maintaining the fundamental character of periodontal ligament.
Practice　： Dentistry
Keywords　： HPLF