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アブストラクト（32巻3/4号：神奈川歯学）
Japanese
| Title ： | ヒト歯胚の発育過程および歯原性遺残上皮におけるbcl-2蛋白発現についての免疫組織学的検討 |
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| Subtitle ： | 原著 |
| Authors ： | 山崎靖, 槻木恵一, 三好代志子, 渡辺是久 |
| Authors（kana） ： | |
| Organization ： | 神奈川歯科大学口腔病理学教室 |
| Journal ： | 神奈川歯学 |
| Volume ： | 32 |
| Number ： | 3/4 |
| Page ： | 260-273 |
| Year/Month ： | 1997 / 12 |
| Article ： | 原著 |
| Publisher ： | 神奈川歯科大学学会 |
| Abstract ： | 「緒言」 bcl-2(B cell lymphoma and leukemia)遺伝子はヒト濾胞性B細胞リンパ腫に高頻度で認められるt(14;18)染色体転座の解析を通して発見された癌遺伝子である. このbcl-2遺伝子は主として8KbのmRNAに転写され, 239個のアミノ酸残基からなる26kの膜蛋白をコードし, この遺伝子産物であるbcl-2蛋白は, 現在スプライシングの違いによりαおよびβの2つの蛋白質に区別されている. この内, 核膜やミトコンドリア膜に発現している分子量24kのαがprogrammed cell deathの一つであるアポトーシス(apoptosis)を抑制する生理機能を有することがHockenbergらやBorzilloらによって明らかにされて以来注目を集め, 癌研究の分野ばかりでなく, 免疫学, 神経学の分野にも新しい一面をもたらしている. このbcl-2遺伝子の特性, すなわちbcl-2蛋白の細胞死抑制活性は, 従来, 細胞増殖の優位性を中心に考えられてきた発癌機構ばかりでなく, 個体の発生途上でみられるアポトーシスを含む種々のprogrammed cell deathの抑制に伴って生ずる組織奇形や病変発生への関与を考えると極めて興味深い. |
| Practice ： | 歯科学 |
| Keywords ： | bcl-2蛋白, ヒト歯胚, 歯原性遺残上皮, アポトーシス |
English
| Title ： | Immunohistochemical Demonstration of bcl-2 Protein in Human Tooth Germ during the Tooth Development and Odontogenic Epithelial Rest |
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| Subtitle ： | Original article |
| Authors ： | Yasushi YAMAZAKI, Keiichi TSUKINOKI, Yoshiko MIYOSHI, Yoshihisa Watanabe |
| Authors（kana） ： | |
| Organization ： | Department of Oral Pathology, Kanagawa Dental College |
| Journal ： | Kanagawa Shigaku |
| Volume ： | 32 |
| Number ： | 3/4 |
| Page ： | 260-273 |
| Year/Month ： | 1997 / 12 |
| Article ： | Original article |
| Publisher ： | Kanagawa Odontological Society |
| Abstract ： | [Abstract] The bcl-2 (B cell lymphoma and leukemia) gene is an oncogene. It was discovered by analysis of t (14 ; 18) chromosome translocation which occurs with high frequency in human follicular B cell lymphoma. The bcl-2 protein, a bcl-2 gene product, is categorized into two types alpha and beta. Alpha bcl-2 protein is known for its physiological function to suppress apoptosis. It expresses in the nuclear membrane and mitochondria of a cell. In the present study, using the TUNEL method, we investigated the possibility that apoptosis is actually implicated in the mechanism of odontogenic epithelial cell death after the formation of teeth. Furthermore, to examine a possible occurrence of bcl-2 protein's function to suppress cell death in the mechanism of occurrence of Malassez's epithelial rests in the jawbone, immunohistochemical investigation was made into the localization of the bcl-2 protein expressed in human tooth germ cells and odontogenic epithelial rests in each developing stages of teeth. The expression of bcl-2 protein was recognized in the tooth germ in the bud stage. In step with the growth of the tooth germ, bcl-2 was continuously expressed in the inner enamel epithelium, Hertwig's epithelial root sheath, ameloblast and stratum intermedium. Bcl-2 protein was also present in Malassez' epithelial rests in the periodontal membrane as well as odontogenic epithelial remnants in the dental sac. On the other hand, in the outer enamel epithelium in the process of atrophy, there appeared signs of apoptosis accompanied by the formation of apoptic bodies. These cells were TUNEL positive. All these findings suggested that after the formation of teeth, apoptotic mechanism could effect cell death of unnecessary odontogenic epithelia. The function attributed to bcl-2 for cell death suppression seemed likely to prolong the life of enamel epithelial cells and promote the formation of enamel. The possible involvement of cell death inhibiting activity by bcl-2 protein in the retention of odontogenic epithelia was also suggested. |
| Practice ： | Dentistry |
| Keywords ： |
