アブストラクト(35巻1号:神奈川歯学)

神奈川歯学

Japanese

Title : 培養ヒト歯根膜細胞の細胞外基質の合成および分解に及ぼす炎症性サイトカインの影響
Subtitle :
Authors : 長谷川英二, 笹栗健一, 山内雅人
Authors(kana) : はせがわえいじ, ささぐりけんいち, やまうちまさと
Organization : 神奈川歯科大学歯科矯正学教室
Journal : 神奈川歯学
Volume : 35
Number : 1
Page : 27-38
Year/Month : 2000 / 3
Article : 原著
Publisher : 神奈川歯科大学学会
Abstract : 歯根膜は咬合時に歯根と歯槽骨の間の緩衝組織として働くほかに, 生理的あるいは病的な歯周組織の改造時に免疫学的および生化学的反応の場として重要な役割を果たしていると考えられている. 歯根膜の炎症を含む歯周炎は, プラークの付着による周囲結合組織の炎症により進行し, 形質細胞, 好中球, マクロファージなどの浸潤に伴い歯周ポケットの形成や歯槽骨の吸収が生じて, 歯の周囲の組織破壊が進行する疾患である. このような歯周組織の破壊には, 単球, 好中球などの血液細胞が発現する炎症性サイトカインとそれらに応答して歯根膜細胞が発現するMMPsなどの基質分解酵素が関与していると考えられている1,2). 炎症における宿主側の応答として, 歯根膜細胞の機能を明らかにすることは, 歯周疾患の機序を解明する上で重要である. MMPファミリーは基質分解酵素の中でも特に重要な酵素で, それぞれが基質特異性を有し, MMPファミリーがすべて存在するとほとんどの細胞外基質の構成物質を分解できると考えられている3~5).
Practice : 歯科学
Keywords : マトリックスメタロプロテアーゼ, IL-1β, TNF-α, Type I collagen

English

Title : Effects of Inflammatory Cytokines on Production of MMPs and Extracellular Matrix Proteins in Cultured Human Periodontal Ligament Fibroblasts
Subtitle :
Authors : Eiji HASEGAWA, Kenichi SASAGURI, Masato YAMAUCHI
Authors(kana) :
Organization : Department of Orthodontics,Kanagawa Dental College
Journal : Kanagawa Shigaku
Volume : 35
Number : 1
Page : 27-38
Year/Month : 2000 / 3
Article : Original article
Publisher : Kanagawa Odontological Society
Abstract : Interleukin-1β (IL-1β) and tumor necrosis factor-α (TNF-α), two potent inflammatory cytokines in periodontitis, were believed to play a significant role in production of matrix metalloproteinases (MMPs), which might be involved in bone loss and connective tissue breakdown. On the other hand, IL-1β had been shown to induce expression of decorin which was a major small proteoglycan participating in the maintenance of periodontal tissue integrity. These cytokines appeared to have a divergent biochemical function in either tissue degradation or regeneration process. To elucidate the molecular mechanisms involved in periodontitis, we examined the effects of IL-1β and TNF-α on expression of MMPs, tissue inhibitors of matrix metalloproteinases (TIMPs), type I collagen and decorin in cultured human periodontal ligament fibroblasts (HPLF). Northern blot analyses showed that each cytokine alone increased the expression of MMP-1 and MMP-2 mRNA and no synergistic effect of these cytokines were detected. Furthermore, Western blot analyses showed that each cytokine increased the production of partially activated MMP-1, while only TNF-α participated in the formation of fully activated MMP-1. Since MMP-3 and plasmin were reported to be important activators of MMP-1, we have examined the effects of inflammatory cytokines on the expression of MMP-3 and urokinase type plasminogen activator (uPA) , an activator of plasminogen to plasmin. Neither IL-1β nor TNF-α participated in the induction of active form of MMP-3 or uPA, suggesting that the enzyme and the activator were not involved in the cascade of MMP-1 activation. Whereas IL-1β or TNF-α alone increased the expression of decorin mRNA, the addition of both cytokines resulted in suppression of the decorin gene expression. The production of type I collagen mRNA was markedly decreased by either cytokine and the synergistic suppressive effect of these cytokines was detected. In conclusion, two potent inflammatory cytokines, IL-1β and TNF-α, could increase the production of MMPs and decorin, and decreased type I collagen expression in HPLF culture. These results suggest that the extracellular matrix degradation in periodontal ligament may facilitate the progression of periodontitis.
Practice : Dentistry
Keywords :