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アブストラクト（35巻1号：神奈川歯学）
Japanese
| Title ： | ヒト歯肉由来線維芽細胞の微細構造学的形態および化学走化性因子発現の経時的変化 |
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| Subtitle ： | |
| Authors ： | 光家由紀子, 東一善*, 木次大介**, 田胡和浩, 出口眞二**, 堀俊雄**, 川瀬俊夫*** |
| Authors（kana） ： | こうけゆきこ, ひがしかずよし, きつぎだいすけ, たごかずひろ, でぐちしんじ, ほりとしお, かわせとしお |
| Organization ： | 神奈川歯科大学歯科保存学講座, *神奈川歯科大学口腔組織学教室, **神奈川歯科大学歯周病学講座, ***神奈川歯科大学歯科生体工学教室 |
| Journal ： | 神奈川歯学 |
| Volume ： | 35 |
| Number ： | 1 |
| Page ： | 51-60 |
| Year/Month ： | 2000 / 3 |
| Article ： | 原著 |
| Publisher ： | 神奈川歯科大学学会 |
| Abstract ： | 歯周組織の再生を確立するには歯周組織の治癒過程において, セメント質, 歯根膜, 歯槽骨の再生が必要となる. 歯周組織再生に対し, 歯根膜由来細胞が果たす役割は大きいと, 報告されている1). なぜならば, 歯根膜組織中には線維芽細胞だけでなく, セメント芽細胞や骨芽細胞の前駆細胞である未分化間葉細胞の存在が認められている2). イヌによるePTFE膜を用いた実験で, 再生された歯根膜組織中にオステオカルシン（OC）強陽性細胞の存在が示されている3). 辻上ら4)はヒト歯根膜由来線維芽細胞（HPLFs）を用い, 3次元培養により作製したスフェロイド中にOCを発現する細胞の存在を明らかにした. また, 現在まで我々は, HPLFsの2次元単層培養法によりHPLFsが培養上清（HPLFs－CM）中に, オートタリン的にHPLFsの接着, 伸展および走化活性を増強させる物質を産生することを明らかにしてきた5,6). HPLFsは血小板由来成長因子（PDGF）, インスリン様成長因子（IGF）や線維芽細胞成長因子（FGF）に走化活性を示すが, 上皮成長因子（EGF）や形質転換成長因子（TGF－β）に対する走化活性は認められていない7,8). |
| Practice ： | 歯科学 |
| Keywords ： |
English
| Title ： | Effect of Culture Period on Ultra-structure and Chemoattractant Expression of Human Gingival Fibroblasts |
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| Subtitle ： | |
| Authors ： | Yukiko KOKE, Kazuyoshi HIGASHI*, Daisuke KITSUGI**, Kazuhiro TAGO, Shinji DEGUCHI**, Toshio HORI**, Toshio KAWASE*** |
| Authors（kana） ： | |
| Organization ： | Department of Operative Dentistry and Endodontics,Kanagawa Dental College, *Oral Histology,Kanagawa Dental College, **Periodontology,Kanagawa Dental College, ***Dental Bioengineering,Kanagawa Dental College |
| Journal ： | Kanagawa Shigaku |
| Volume ： | 35 |
| Number ： | 1 |
| Page ： | 51-60 |
| Year/Month ： | 2000 / 3 |
| Article ： | Original article |
| Publisher ： | Kanagawa Odontological Society |
| Abstract ： | Motility and adherence of human periodontal ligament fibroblasts (HPLFs) on root surface are important of tissue remodeling during periodontal regeneration. The purpose of this study was to evaluate the expression of chemoattractant of human gingival fibroblasts conditioned medium (HGFs-CM) to HPLFs at the different periods of incubation. Chemotactic activity and protein concentrations of HGFs-CMs which were harvested after 3, 7, 14, 21, and 28 day of incubation, were measured respectively. At harvest HGFs-CMs, the morphological structures of cultured cells were observed by phase-contrast microscope and by transmission electron microscope. Total protein concentrations of HGFs-CM was increased dependent on culture periods. Chemotactic activity of HGFs-CM to HPLFs was increased from 3 to 21 day and then seemed to plateau at 28 day of incubation. Those specimens were observed by the electron microscope, numerous free ribosomes were present in the cytoplasm of HGFs on 3 day, and primarry cilia were detected in HGFs on 7 to 21 day of incubation. Extracellular matrix substratum of HGFs was increased dependent on incubation period. These results suggested that 7 and 14 day of incubation were best times for harvest of chemoattractant, because of these HGFs-CM contained higher specific activity of chemotaxis compared with other incubation times. HGFs on 3 to 21 day in vitro were examined by the transmission electron microscope. HGFs on 3 day were spindle in shape and contained numerous free ribosomes. Therefore, it seemed that HGFs were immature, but HGFs on 14 to 21 day were seen as mature type. Because, these cells had well developed cytoplasmic organelles, particulary wide cisterna of rough surfaced endoplasmic retucula, and primary cilia with other centrioles. These observation seem to parallel histochemical results in this study. |
| Practice ： | Dentistry |
| Keywords ： | cell culture, fibroblast, chemotaxis, ultrastructure |
