アブストラクト(38巻1号:神奈川歯学)

神奈川歯学

Japanese

Title : Porphyromonas gingivalis lipid Aのマウス細胞に対する免疫生物活性‐炎症性サイトカイン産生および破骨細胞分化誘導能‐
Subtitle :
Authors : 谷昇, 渡辺清子, 熊田秀文
Authors(kana) :
Organization : 神奈川歯科大学口腔細菌学教室
Journal : 神奈川歯学
Volume : 38
Number : 1
Page : 10-20
Year/Month : 2003 / 3
Article : 原著
Publisher : 神奈川歯科大学学会
Abstract : Porphyromonas gingivalis (P. gingivalis)は偏性嫌気性の黒色色素産生グラム陰性桿菌であり, 成人性歯周疾患との関わりが強く示唆されている1~3). 本菌の歯周病原因子としては, 細菌の菌体成分や代謝産物, 酵素などによる宿主組織への直接的破壊作用の他, 宿主の免疫応答を惹起する生物活性が生体に様々な影響を与える間接作用とが挙げられる4~6). グラム陰性菌の外膜成分であるリポ多糖体(Lipo-polysaccharide;LPS)は多彩な生物活性を有しており7~9), P. gingivalisのLPSは腸内細菌のLPSと比較して発熱原性や致死毒性あるいは局所シュワルツマン反応惹起能など, いわゆる内毒素活性と呼ばれる活性が弱い反面, 線維芽細胞に対する活性や骨吸収活性に関してはむしろ腸内細菌LPSよりも強い活性を示すこと, さらにLPS不応答性マウスの細胞に対しても活性を示すなどの特徴を有することが明らかにされている10~14).
Practice : 歯科学
Keywords : Porphyromonas gingivalis, Lipopolysaccharide(LPS), サイトカイン, 破骨細胞

English

Title : Involvement of Lipid A from Porphyromonas gingivalis in Production of Inflammatory Cytokines and Osteoclast Differentiation in Mice
Subtitle :
Authors : Noboru TANI, Kiyoko WATANABE, Hidefumi KUMADA
Authors(kana) :
Organization : Department of Oral Microbiology, Kanagawa Dental College
Journal : Kanagawa Shigaku
Volume : 38
Number : 1
Page : 10-20
Year/Month : 2003 / 3
Article : Original article
Publisher : Kanagawa Odontological Society
Abstract : Porphyromonas gingivalis is thought to be one of periodontopathic bacteria that have been strongly associated with adult periodontitis. Lipopolysaccharide (LPS) of P. gingivalis expresses various activities that are directly connected to the periodontal diseases of bone resorption and induction of various cytokines, therefore, it is assumed to play an important role in periodontal tissue breakdown. In this study, we investigated the immunobiological activities of lipid A, an active center of LPS, isolated from P. gingivalis to induce inflammatory cytokines and osteoclast differentiation in mice. The P. gingivalis lipid A was found to induce IL-1α, IL-6, and TNF-αrelease from peritoneal macrophages in LPS-responsive C3H/HeN mice. The activity was, however, about 10-fold less than that of Salmonella typhimurium SL684 lipid A used as a control. Likewise, the lipid A exhibited explicit ability to elicit the inflammatory cytokines in LPS-nonresponsive C3H/HeJ mice, which may be explained by its unique structure. In order to clarify the induction of osteoclast differentiation by P. gingivalis lipid A, the lipid A was added to co-cultured cells of MC3T3-G2/PA6 and mice bone marrow cells in the presence or absence of lα, 25(OH)2D3. The colonies containing tartrate-resistant acid phosphatase (TRAP) positive cells were formed with the addition of the lipid A to the co-culture cells in absence of lα, 25 (OH)2D3. Moreover, the addition of the lipid A increased the number of multinuclear cells formation in the presence of 1α, 25(OH)2D3. These results suggest that the lipid A from P. gingivalis is the potent activator that stimulates inflammatory cytokines production and osteoclast differentiation, which may therefore play an important role in the development of periodontal diseases.
Practice : Dentistry
Keywords : Porphyromonas gingivalis, Lipopolysaccharide (LPS)