アブストラクト（41巻1号：神奈川歯学）

神奈川歯学

Japanese

Title　： 骨芽細胞分化とノジュール形成におけるヒト歯根膜線維芽細胞とラット骨髄細胞の相互作用の効果について
Subtitle　：
Authors　： 新井信明*, 根岸秀幸*,**, 小園 知**, 川瀬俊夫*,**
Authors（kana）　：
Organization　： *神奈川歯科大学自然科学講座歯科生体工学分野, **神奈川歯科大学高次口腔科学研究所
Journal　： 神奈川歯学
Volume　： 41
Number　： 1
Page　： 17-30
Year/Month　： 2006 / 6
Article　： 原著
Publisher　： 神奈川歯科大学学会
Abstract　： 緒言 歯根膜は歯根膜側の歯槽骨およびセメント質と同様に歯小嚢由来である1～3). 歯根膜の主たる構成細胞は線維芽細胞であるが, 明らかに歯肉の線維芽細胞とは異なる特徴を持っている. すなわち歯根膜の線維芽細胞は骨の標識であるalkaline phosphatase(ALPase)活性が高いことから, 骨芽細胞様線維芽細胞(osteo-blastic fibroblasts)と, 報告されている4～9). しかし, 歯根膜の線維芽細胞が石灰化能を持っているかどうかは, 議論の余地ある10, 11). 歯根膜は生理的に非石灰化の結合組織であって, 病的なankylosisによって初めて石灰化する12～14). 近年組織再生において胚性幹細胞ばかりでなく組織幹細胞が注目され, どのような組織や器官においても幹細胞が存在していると考えられている15, 16). Seo BMらは成人の第3大臼歯の歯根膜に多機能分化能を持った幹細胞(periodontal ligament stem cells：PDLSCs)を同定した. すなわち, PDLSCsは間葉系幹細胞の標識のSTRO-1とCD146/MUC18を発現し, cementoblast-like cells, adipocytesおよびコラーゲン産生細胞に分化する, と報告している17, 18).
Practice　： 歯科学
Keywords　： 歯根膜線維芽細胞, 骨髄細胞, 骨系細胞分化, 石灰化抑制

English

Title　： Effect of Interaction of Human Periodontal Ligament Fibroblasts and Rat Bone Marrow Cells on the Osteogenic Differentiation and Nodule Formation
Subtitle　：
Authors　： Nobuaki ARAI*, Hideyuki NEGISHI*,**, Satoru OZONO**, Toshio KAWASE*,**
Authors（kana）　：
Organization　： *Department of Natural Science, Division of Dental Bioengineering, Kanagawa Dental College, **Institute of Frontier Oral Science, Kanagawa Dental College
Journal　： Kanagawa Shigaku
Volume　： 41
Number　： 1
Page　： 17-30
Year/Month　： 2006 / 6
Article　： Original article
Publisher　： Kanagawa Odontological Society
Abstract　： Periodontal ligament is a non-calcified connective tissue that connects cementum and alveolar bone to maintain and support teeth in situ and maintain homoeostasis. Periodontal ligament fibroblasts have osteoblast-like properties, including the expression of bone-associated marker such as alkaline phosphatase (ALPase). To demonstrate the role of bone marrow cell-periodontal ligament fibroblast communication during process of tissue regeneration, we investigated that human periodontal ligament-derived fibroblasts (HPLF) contact directly or indirectly with rat bone marrow cells (RBMC). The confluent HPLF that were contacted directly and cultured with RBMC for 7 days were examined for the cell growth and ALPase activity levels. The expression of ALPase, osteopontin (OPN) and integrinβ1 mRNAs were analyzed by the method of reverse transcriptase-polymerase chain reaction (RT-PCR) using primers that could be detected human mRNAs. After 7 days, co-cultured HPLF expressed greater amounts of mRNA for ALPase mRNA and OPN mRNA than did HPLF cultured alone. HPLF may differentiate the osteogenic cells by direct cell-to-cell contact with stem cell-like RBMC. RBMC co-cultured with insert HPLF were cultured in differential medium, which composed of α-Minimum Essential Medium (α-MEM) supplemented with 10 nM dexamethasone (Dex) and 10 mM β-glycerophosphate (β-GP). After 14 days, co-cultured RBMC produced bone nodules that were significantly decreased in number (271.2±56.4 nodules versus 114.8±11.8 nodules per well, p≦0.05) than those of RBMC cultured alone. RBMC co-cultured with insert HPLF inhibited the expression of mRNA for osteogenic markers of bone sialoprotein (BSP) (27.4%) and OPN (21.4%) than did RBMC cultured alone. These results indicate that the osteogenic properties of periodontal ligament fibroblasts may induced by stem cells, and periodontal ligament fibroblasts may maintain the non-calcified connective tissue. The authors hypothesize that this effect was due to paracrine signaling from periodontal ligament fibroblasts.
Practice　： Dentistry
Keywords　：