アブストラクト(46巻1号:神奈川歯学)

神奈川歯学

Japanese

Title : LIPUS照射による歯周組織由来細胞および間葉系幹細胞の増殖と分化への影響
Subtitle : 原著
Authors : 眞野壮一郎*1, 宗正憲和*1, 岩崎和人*1, 高橋弘行*1, 田胡和浩*1, 川瀬俊夫*2, 出口眞二*1
Authors(kana) :
Organization : *1神奈川歯科大学大学院歯学研究科歯周病学講座, *2神奈川歯科大学自然科学講座歯科生体工学分野
Journal : 神奈川歯学
Volume : 46
Number : 1
Page : 37-45
Year/Month : 2011 / 6
Article : 原著
Publisher : 神奈川歯科大学学会
Abstract : 「緒言」近年, 超音波による物理療法は, 低侵襲で治療効果が高い治療法として多種多様な研究が行われている. その中で低出力パルス超音波(Low-intensity pulsed ultrasound:LIPUS)は微弱な超音波エネルギーを応用した治療法1-3)であり, 整形外科領域において効率的な治療法として応用されている. LIPUSによる骨形成の作用は, ウサギ腓骨骨折後の治癒および大腿骨骨欠損の修復に対する効果として1983年にDuarte4)により初めて報告された. 1994年にHekman5)は脛骨皮下骨折に対して, 1997年にはKristianen6)らも, 撓骨遠位端骨折に対して治癒期間の短縮に有効であることを報告した. その後, 骨折治療への応用に特化した治療技術や治療機材が開発され, 整形外科領域では遷延治癒や偽関節, 新鮮骨折の治療に保険適用されている. 骨折治療における超音波の機械的な振動は, 非侵襲的に外部より生体深部に投入される. 骨は, 力学的環境に応じてその三次元構造を適応させる7).
Practice : 歯科学
Keywords : LIPUS, 細胞分化, 歯周組織由来細胞

English

Title : Effects of Low-intensity Pulsed Ultrasound on Proliferation and Differentiation of Periodontium-Derived Cells and Rat Bone Marrow Cells
Subtitle :
Authors : Soichiro MANO*1, Norikazu MUNEMASA*1, Kazuto IWASAKI*1, Hiroyuki TAKAHASHI*1, Kazuhiro TAGO*1, Toshio KAWASE*2, Shinji DEGUCHI*1
Authors(kana) :
Organization : *1Department of Periodontology, Kanagawa Dental College Graduate School, *2Department of Natural Science, Division of Dental Bioengineering, Kanagawa Dental College
Journal : Kanagawa Shigaku
Volume : 46
Number : 1
Page : 37-45
Year/Month : 2011 / 6
Article : Original article
Publisher : Kanagawa Odontological Society
Abstract : Low-intensity pulsed ultrasound (LIPUS) accelerates wound healing of experimental alveolar bone defects, but the exact mechanism is unknown. The purpose of this study was to investigate the effect of LIPUS stimulation on the proliferation and differentiation of periodontium-derived cells and rat bone-marrow cells (RBMCs).Human gingival fibroblasts (HGFs), Human periodontal ligament fibroblasts (HPLFs), and Human alveolar bone periosteal cells (HABPCs) were cultured from gingival specimens and periosteal specimens obtains from patients undergoing gingival flap surgery. RBMCs was derived from the femurs of 7-week-old male Fisher rats. Cells were cultured in 24 well culture dishes, were subjected two different ultrasound exposure (frequency of 3 MHz or 5 MHz, pulse intensity = 160 mW/ cm2, spatial and temporal average [SATA] for 15 min per day) for 3, 7, 10, and 14 days. The assays performed were cell proliferation, alkaline phosphatase (ALP) activity, and mineralization activity (using the von Kossa stain). Cell proliferation was not stimulated in any of the four cell types. ALP activity in HPLFs and RBMCs significantly increased with 5 MHz at 10, 14 days and 7, 10 days compared with 3 MHz and the control. LIPUS exposure with 5 MHz at 7 days significantly increased the expression level of osteopontin (OP) in HABPCs compared with 3 MHz. We identified mineralizing cells in vitro that immunostained positively for OP and osteocalcin in RBMCs cultured with by supplementing the culture medium with beta-glycerophosphate and dexamethasone at 7 days LIPUS exposure with 5 MHz ultrasound. 5 MHz ultrasound induces significantly enhanced positive staining area for von Kossa compared with the control in RBMCs cultured in an osteogenic medium.
Practice : Dentistry
Keywords :