アブストラクト（49巻2号：神奈川歯学）

神奈川歯学

Japanese

Title　： ラット顎下腺主導管における刷子細胞に関する免疫組織化学的研究
Subtitle　： 原著
Authors　： 加藤智弘, 河田亮, 東一善, 高橋理
Authors（kana）　：
Organization　： 神奈川歯科大学大学院神経発生組織学講座
Journal　： 神奈川歯学
Volume　： 49
Number　： 2
Page　： 101-110
Year/Month　： 2014 / 12
Article　： 原著
Publisher　： 神奈川歯科大学学会
Abstract　： 「緒言」顎下腺主導管の上皮細胞はラットにおいて円柱型および立方型の基底細胞より構成される多列上皮である. 電子顕微鏡を用いた超微構造学的な研究によると, 多列上皮を構成する多数が上皮細胞に加えて, 少数の刷子細胞と呼ばれる多数のフィラメントを含む大型の微絨毛を有する細胞が報告され, さらにごく少数の基底顆粒細胞の存在も報告される. この刷子細胞は別名, 毛束細胞や小胞細胞とも呼ばれ, ラットの気管上皮にて電子顕微鏡下に最初に観察されて以来, 呼吸器系, 消化器系 (胆嚢, 胆管, 膵管) など様々な上皮組織でその存在が報告されてきた. この中で特に消化器系にみられる刷子細胞はパラクリン (傍分泌) 作用を有する可能性が指摘され, 伝達物質として一酸化窒素が候補に挙げられるが, その実験的な裏付けは報告されていない. 口腔の唾液腺主導管においては刷子細胞の存在が電子顕微鏡下に報告され, 円柱型もしくは樽型の形態とともに顎下腺主導管の上皮中に散在するといわれる.
Practice　： 歯科学
Keywords　： 刷子細胞, 顎下腺主導管, PGP9.5, VIP

English

Title　： Immunohistochemical study of the brush cells in main excretory duct of the rat submandibular gland
Subtitle　：
Authors　： Tomohiro KATO, Akira KAWATA, Kazuyoshi HIGASHI, Osamu TAKAHASHI
Authors（kana）　：
Organization　： Division of Histology, Embryology and Neuroanatomy, Kanagawa Dental University, School of Dentistry
Journal　： Kanagawa Shigaku
Volume　： 49
Number　： 2
Page　： 101-110
Year/Month　： 2014 / 12
Article　： Original article
Publisher　： Kanagawa Odontological Society
Abstract　： [Abstract] Recent histochemical and ultrastructural studies have indicated that primary saliva is modified in the main excretory duct (MED) of the salivary glands by secretion of K+ and HCO3- and by absorption of Na+ and Cl-. The epithelium-lined MED of the rat submandibular gland is pseudostratified, consisting of many columnar and cuboidal cells, brush cells (BCs) and a few granulated cells. BCs have been identified in various organs, such as the stomach, colon and gallbladder in the digestive system, and the lungs in the respiratory system. Compared to other epithelial cells, BCs are considered to possess longer and thicker microvilli. These bundles of filaments have been observed to extend from the supranuclear region of the BC to the top of each microvillus. Although many morphological features of BCs have been reported, the function of BCs has not yet been clarified. In addition, experimental evidence on the functions of BCs is also lacking due to the difficulty in detecting BCs, which represent a minor component of epithelial cells of the MED. Distinguishing BCs from other epithelial cells in the MED under light microscopy is difficult. Therefore, this study investigated epithelial cells of the MED using immunohistochemical and immunoelectron microscopic techniques that employed primary antibodies against protein gene product 9.5 (PGP 9.5), cytokeratin 18 (CK18), vasoactive intestinal peptide (VIP), and neurofilament (NF). CK18 immunoreactivity was shown to exist in all epithelial cells of the MED, so antibody against CK18 was not suitable as a specific marker for BCs in the MED under light microscopy. On the other hand, PGP 9.5 immunoreactivity was observed in epithelial cells in the MED. Moreover, VIP immunoreactivity was shown to exist in PGP 9.5 immunoreactive epithelial cells, and NF immunoreactivity was observed in PGP immunoreactive cells. By employing immunoelectron microscopic techniques, both PGP 9.5 and VIP immunoreactivity were observed on the bundles of filaments and cytoplasm of BCs. From these results, it appeared that PGP 9.5, VIP and NF immunoreactivity could be used as specific markers of BCs under light microscopy. PGP 9.5 and VIP immunoreactivity were observed in the taste buds of rats which resembled BCs in overall shape. Since BCs showed similar ultrastructural and immunohistochemical characteristics to taste bud cells, it is possible that BCs have a chemosensory or somatosensory function.
Practice　： Dentistry
Keywords　： PGP9.5, VIP