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アブストラクト（51巻2号：神奈川歯学）
Japanese
| Title ： | 低出力超音波パルスLIPUSを照射したヒト歯槽骨骨膜由来スフェロイドに関する超微細構造学的研究 |
|---|---|
| Subtitle ： | 原著 |
| Authors ： | 宮城直美, 川津布美*, 東一善*, 前田信吾, 河田亮, 高橋理** |
| Authors（kana） ： | |
| Organization ： | 神奈川歯科大学大学院歯学研究科口腔科学講座神経組織発生学分野, *川津歯科医院, **主任 |
| Journal ： | 神奈川歯学 |
| Volume ： | 51 |
| Number ： | 2 |
| Page ： | 71-81 |
| Year/Month ： | 2016 / 12 |
| Article ： | 原著 |
| Publisher ： | 神奈川歯科大学学会 |
| Abstract ： | 「緒言」最近, 川津らはヒト歯槽骨骨膜由来細胞(HABPCs)のスフェロイドを作製した後に低出力超音波パルス(Low-intensity Pulsed Ultrasound(LIPUS))を照射し, スフェロイド内の細胞形態およびオステオポンチン(OP)およびオステオカルシン(OC), すなわち骨基質タンパクの発現に与えるLIPUSの影響を経時的に検索した結果, 細胞構築および細胞形態により分類別された層構造がスフェロイドに認められ照射近位部, 照射遠位部, コントロールの順で経時的に層構造は不明瞭になり, さらに全てのスフェロイドに深層から中層, 表層の順にOPないしはOCに対する免疫陽性細胞の発現を報告した. またLIPUS照射近位部で早期にOP, OCの発現と細胞の索状構造が認められ, スフェロイド内におけるHABPCsの分化促進と細胞構築の変化が生ずることも示唆された. |
| Practice ： | 歯科学 |
| Keywords ： | 低出力超音波パルス, スフェロイド, ヒト歯槽骨骨膜由来細胞, 電子顕微鏡 |
English
| Title ： | Ultrastructural study of human alveolar bone periosteal cell spheroids upon exposure to Low-intensity Pulsed Ultrasound (LIPUS) |
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| Subtitle ： | |
| Authors ： | Naomi MIYAGI, Fumi KAWATSU*, Kazuyoshi HIGASHI*, Shingo MAEDA, Akira KAWATA, Osamu TAKAHASHI |
| Authors（kana） ： | |
| Organization ： | Department of Neuroanatomy, Histology, and Embryology, Kanagawa Dental University Graduate School, *Kawatsu Dental Clinic |
| Journal ： | Kanagawa Shigaku |
| Volume ： | 51 |
| Number ： | 2 |
| Page ： | 71-81 |
| Year/Month ： | 2016 / 12 |
| Article ： | Original article |
| Publisher ： | Kanagawa Odontological Society |
| Abstract ： | [Abstract] Previously, low-intensity pulsed ultrasound (LIPUS) was reported to accelerate periodontal wound healing in experimental alveolar bone defects in dogs, however, the exact mechanism is still unknown. This study attempted to determine how daily LIPUS stimulation might affect morphological and cytoarchitectonic changes of the cells and bone matrix protein expression in spheroids of human alveolar bone periosteal cells. Histological (toluidine blue), immunohistochemical, and ultra-structural analyses (osteopontin (OP) and osteocalcin (OC)) of spheroid cells were conducted after 1, 3, 7, and 14 days of culture with daily LIPUS stimulation. Layered structures, distinguished by the directionality of the cells, were observed in the spheroids from day 1, with the layered structures becoming indistinct over time in the order of the irradiated side, non-irradiated side, and control. Immuno-histochemically positive cells indicating OP and OC were observed in the order of the core, middle layer, and outer layer of the spheroids. The shapes of the cell bodies were columnar, cuboidal, and flattened, respectively. Cells at the core part of the spheroids showed electron-dense granules in their cytoplasm and in matrix between their cell bodies. Furthermore, immuno-positive cells for OC and OP were encountered over time in the order of the irradiation side, non-irradiation side, and controls. In addition, cells with funicular structure were observed in the core from the outer layer of the spheroids on the irradiated side from day 1. These findings show that LIPUS irradiation promotes cell differentiation and changes the cell direction within spheroids. |
| Practice ： | Dentistry |
| Keywords ： |
