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アブストラクト（53巻1/2号：神奈川歯学）
Japanese
| Title ： | Actinomyces naeslundii 線毛による口腔バイオフィルム形成機構の解明 |
|---|---|
| Subtitle ： | 神奈川歯科大学学会 第52回総会宿題報告 |
| Authors ： | 佐藤武則 |
| Authors（kana） ： | |
| Organization ： | 神奈川歯科大学大学院歯学研究科口腔科学講座口腔分子細胞制御学分野・助教 |
| Journal ： | 神奈川歯学 |
| Volume ： | 53 |
| Number ： | 1/2 |
| Page ： | 69-73 |
| Year/Month ： | 2018 / 12 |
| Article ： | 報告 |
| Publisher ： | 神奈川歯科大学学会 |
| Abstract ： | 「緒言」 歯周炎は多種類の口腔内細菌が混在するデンタルプラーク(口腔バイオフィルム)による感染症である. 口腔バイオフィルムはStreptococcus属やActinomyces属などのグラム陽性菌が唾液糖タンパク質由来のペリクルを介して歯面や歯肉溝に初期定着し, 後期定着細菌として歯周病原性をもつPorphyromonas gingivalisやTreponema denticolaなどのグラム陰性菌と共凝集することで成熟することが知られている. このような特徴をもつ口腔バイオフィルムにおいて, グラム陽性菌Actinomyces naeslundiiは口腔バイオフィルムの中核的存在を担い, 歯周病原細菌が定着可能な環境を整備する役割があると言われている. A. naeslundiiはこれまで健康な人に多い口腔常在菌であり, う蝕や歯肉炎の発症に関与するものの, 慢性歯周炎患者ではA. naeslundiiの細菌数が減少することから, 本菌の歯周病原性に関しては注目されてこなかった. |
| Practice ： | 歯科学 |
| Keywords ： | Actinomyces naeslundii, 口腔バイオフィルム, 線毛, 歯周炎 |
English
| Title ： | Actinomyces naeslundii elucidate mechanism of oral biofilm formation by pili[Machine Translation] (Actinomyces naeslundii 線毛による口腔バイオフィルム形成機構の解明) |
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| Subtitle ： | |
| Authors ： | Takenori SATO |
| Authors（kana） ： | |
| Organization ： | Division of Oral Molecular and Cellular Biochemistry, Department of Oral Science, Graduate School of Dentistry, Kanagawa Dental University |
| Journal ： | Kanagawa Shigaku |
| Volume ： | 53 |
| Number ： | 1/2 |
| Page ： | 69-73 |
| Year/Month ： | 2018 / 12 |
| Article ： | Report |
| Publisher ： | Kanagawa Odontological Society |
| Abstract ： | [Abstract] The purpose of this study was analyzed the influence of temperature and bacterial nutrition with A. naeslundii biofilm formation. A. naeslundii T14V was grown in brain heart infusion (BHI) broth supplemented with yeast extract (YE) with various concentrations, divided into the following six groups under anaerobic condition. Group A was cultured with 3.7% BHI and 0.5% YE at 37℃, Group B was cultured with 1.85% BHI and 0.5% YE at 37℃, Group C was cultured with 1.85% BHI and 0.25% YE at 37℃, Group D was cultured with 3.7% BHI and 0.5% YE at 39℃, Group E was cultured with 1.85% BHI and 0.5% YE at 39℃ and Group F was cultured with 1.85% BHI and 0.25% YE at 39℃. The bacterial growths were monitored by using a spectrophotometer at 550 nm for 0 to 48 hours. To evaluate bacterial biofilm formation, the biofilm was performed on the 24-well polystyrene plates with the sterilized coverslips. After incubation, the biofilm was removed by sodium hydroxide solution. Biofilm assay was evaluated the absorbance of the inoculation containing removed biofilm. Visual coaggregation assay was scored by mixed with S. gordonii bacterial suspension or P. gingivalis bacterial suspension against A. naeslundii bacterial suspension. To determine the influence of temperature and medium composition, A. naeslundii bacterial protein were analyzed by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE). The growth of A. naeslundii at 37℃ and 39℃ were no difference, whereas bacterial cells were decreased by the low concentrations of medium composition. Coaggregation activity of A. naeslundii was decreased cultured at 39℃ as compared with cultured at 37℃. Moreover, the whole cell proteins of cultured at 39℃ were also decreased in a dose-dependent manner. These results suggested that the pathogenesis of A. naeslundii may deal with the changes of culture conditions and alter the structures of oral biofilm. |
| Practice ： | Dentistry |
| Keywords ： | Actinomyces naeslundii |
