アブストラクト（54巻2号：神奈川歯学）

神奈川歯学

Japanese

Title　： ラット顎下腺の腺房, 主導管, 導管周囲細動脈に分布する神経活性物質およびシナプトフィジン免疫陽性神経終末に関する免疫組織化学的研究
Subtitle　： 原著
Authors　： 大西宏祐1), 小口岳史2), 平野航1), 杉村太丈3), 前田信吾1), 河田亮1), 尾之上さくら4), 東一善1), 高橋理1)
Authors（kana）　：
Organization　： 1)神奈川歯科大学大学院歯学研究科口腔科学講座神経組織発生学分野, 2)神奈川歯科大学総合教育部, 3)神奈川歯科大学大学院歯学研究科口腔統合医療学講座歯科矯正学分野, 4)関東学院大学理工学部
Journal　： 神奈川歯学
Volume　： 54
Number　： 2
Page　： 69-75
Year/Month　： 2019 / 12
Article　： 原著
Publisher　： 神奈川歯科大学学会
Abstract　： 「緒言」顎下腺は顎下三角に存在し, 上面は下顎骨の顎下腺窩と顎舌骨筋に接し, 下面は広頸筋に覆われる. 顎下神経節は顎下三角に位置し, ラットでは顎下腺主導管の長軸に沿う結合組織において, 顎下腺の門より咬筋後縁まで柱状の形態を呈する. 過去の研究で, 顎下神経節中においては多くの神経活性物質の存在が報告されている. 免疫組織化学的研究ではcalcitonin gene-related peptide, substance P, vasoactive intestinal polypeptide (VIP), neuropeptide-Y(NPY), enkephalin, 5-hydroxytryptamine(5-HT, serotonin)等の神経活性物質の存在が明らかとなっている. われわれはこの中で, VIP, NPY, 5-HT に着目して, 唾液腺の分泌, 吸収への関与を調査してきた. VIPは消化管の分泌や運動を調節するペプチドホルモンであり, 口腔領域においては顎下腺および口唇の血管拡張に関与する.
Practice　： 歯科学
Keywords　： 顎下腺, neuropeptide Y, vasoactive intestinal polypeptide, serotonin

English

Title　： Immunohistochemical study of synaptophysin- immunopositive nerve endings in acinar portion, main excretory ducts and arteries surrounding duct of rat submandibular gland
Subtitle　：
Authors　： Kosuke ONISHI1), Takeshi OGUCHI2), Wataru HIRANO1), Takahiro SUGIMURA3), Shingo MAEDA1), Akira KAWATA1), Sakura ONOUE4), Kazuyoshi HIGASHI1), Osamu TAKAHASHI1)
Authors（kana）　：
Organization　： 1)Division of Histology, Embryology and Neuroanatomy, Department of Oral Science, Graduate School of Dentistry, Kanagawa Dental University, 2)Division of Curriculum Development, Kanagawa Dental University, 3)Division of Orthodontics Department of Oral Interdisciplinary Medicine Graduate School of Dentistry, Kanagawa Dental University, 4)College of Science and Engineering, Kanto Gakuin University
Journal　： Kanagawa Shigaku
Volume　： 54
Number　： 2
Page　： 69-75
Year/Month　： 2019 / 12
Article　： Original article
Publisher　： Kanagawa Odontological Society
Abstract　： [Abstract] Previous submandibular ganglion studies have reported the presence of neuropeptide Y (NPY) and vasoactive intestinal peptide (VIP)-immunopositive nerve fibers as well as 5-hydroxytryptamine (5-HT, serotonin)- immunopositive cells in epithelial cells of the main excretory duct. These studies have also demonstrated the association of these entities with secretion from the submandibular gland and modification of the saliva in the main excretory duct. However, a detailed investigation has yet to be conducted on the distribution of nerve endings that made contacts to submandibular gland acini or the main excretory duct. In this study, we therefore sought to determine the morphological characteristics and distribution of nerve endings in the submandibular gland acini, the main excretory duct, and arteries surrounding the duct. Immunostaining images observed under confocal laser microscopy showed that most nerve endings distributed to the main excretory duct and nearby arteries exhibited a VIP-immunopositive response. NPY-immunopositive nerve endings were common in the acinus. In addition, synaptophysin (SPN)-immunopositive nerve endings were most commonly observed in the acinus and arteries around the main excretory duct and the main excretory duct itself. Based on these results, it was concluded that the type and number of nerve fibers projected in the main excretory duct and acini differ. In the future, the search for receptors expressed in the main excretory duct and acini cells and the changes in salivary secretion by VIP, NPY and 5-HT administration experiments will lead to the elucidation of the salivary secretion mechanism.
Practice　： Dentistry
Keywords　： neuropeptide Y, vasoactive intestinal polypeptide, serotonin