アブストラクト（54巻2号：神奈川歯学）

神奈川歯学

Japanese

Title　： 神経線維束中の神経細胞とラット顎下神経節の免疫組織化学的研究
Subtitle　： 原著
Authors　： 平野航1), 東一善1), 河田亮1), 大西宏祐1), 杉村太丈2), 小口岳史3), 前田信吾1), 尾之上さくら4), 高橋理1)
Authors（kana）　：
Organization　： 1)神奈川歯科大学大学院歯学研究科口腔科学講座神経組織発生学分野, 2)神奈川歯科大学大学院歯学研究科口腔統合医療学講座歯科矯正学分野, 3)神奈川歯科大学総合教育部, 4)関東学院大学理工学部
Journal　： 神奈川歯学
Volume　： 54
Number　： 2
Page　： 76-84
Year/Month　： 2019 / 12
Article　： 原著
Publisher　： 神奈川歯科大学学会
Abstract　： 「緒言」顎下神経節はヒトでは顎下三角に, ラットでは顎下腺主導管の長軸に沿って, 顎下腺門付近から遠位部に存在している. Ng YKらがその形態について研究を行い, 顎下神経節の機能についての研究が行われるようになってきている. 神経活性物質に関しての研究も行われており, vasoactive intestinal polypeptide(VIP), calcitonin gene-related peptide(CGRP), ニューロペプチドY(NPY)やserotonin(5-HT)等が顎下神経節中に存在している. VIPは中枢神経や消化管では電解質の分泌, 吸収, 血流調節に関与している. 顎下腺主導管上皮中の刷子細胞にVIP免疫陽性反応があり, また同様に主導管上皮中にセロトニン陽性を示す細胞が存在し, 合わせて顎下神経節にもセロトニンの存在を見出している. さらに顎下腺ではVIPは血管の拡張に関与していると報告されている.
Practice　： 歯科学
Keywords　： 顎下腺, neuropeptide Y vasoactive intestinal polypeptide, serotonin

English

Title　： An immunohistochemical study of rat submandibular ganglion and nerve cells observed in the bundles
Subtitle　：
Authors　： Wataru HIRANO1), Kazuyoshi HIGASHI1), Akira KAWATA1), Kosuke ONISHI1), Takahiro SUGIMURA2), Takeshi OGUCHI3), Shingo MAEDA1), Sakura ONOUE4), Osamu TAKAHASHI1)
Authors（kana）　：
Organization　： 1)Division of Histology, Embryology and Neuroanatomy, Department of Oral Science, Graduate School of Dentistry, Kanagawa Dental University, 2)Division of Orthodontics, Department of Oral Interdisciplinary Medicine, Graduate School of Dentistry, Kanagawa Dental University, 3)Division of Curriculum Development, Kanagawa Dental University, 4)College of Science and Engineering, Kanto Gakuin University
Journal　： Kanagawa Shigaku
Volume　： 54
Number　： 2
Page　： 76-84
Year/Month　： 2019 / 12
Article　： Original article
Publisher　： Kanagawa Odontological Society
Abstract　： [Abstract] We have studied immunohistochemically for physiologically active substances, such as vasoactive intestinal peptide (VIP) and neuropeptide Y (NPY), in rat submandibular ganglion cells. We have also observed the ultrafine structure of the submandibular ganglia using light and electronic microscopy and have reported the localization of VIP and NPY in these ganglia. We have also identified ganglion neurons in the nerve fiber bundle near the ganglion. Therefore, in this study, we performed ultrafine structural analysis and immunohistochemistry with these ganglion neurons in the same way as rat submandibular ganglion neurons. The submandibular gland and surrounding tissues of male Wistar rats were excised, fixed, and embedded in resin. Serial sections were prepared, stained using toluidine blue, and observed with a light microscope. Ultrathin sections were prepared by the same fixation and embedding method, and then observed with an electron microscope. In addition, immunostaining for VIP, NPY, 5-HT(serotonin), protein gene product 9.5 (PGP 9.5), etc. was performed and observed with a confocal laser microscope. Ganglion cells located outside the submandibular ganglion are distributed along the main conduit, but morphological features of the soma and mantle cells were similar to those of submandibular ganglion neurons. Most cell bodies found in the vicinity of the ganglia were VIP immunopositive, and NPY-immunopositive cells were rare. Many VIP-immunopositive cells were observed around the main conduit. VIP-immunopositive cells, which are frequently observed in the vicinity of the submandibular gland main duct, are thought to control cell functions such as secretion and absorption of the main duct.
Practice　： Dentistry
Keywords　： neuropeptide Y vasoactive intestinal polypeptide, serotonin